Transporter

Details

Name
Transporter
Synonyms
Not Available
Gene Name
snf
Organism
Aquifex aeolicus (strain VF5)
Amino acid sequence
>lcl|BSEQ0012739|Transporter
MEVKREHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMW
IEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAI
KFLVGLVPEPPPNATDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINV
SILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKD
PGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSISIP
AAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIM
QPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTEL
IIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLVVWAREYIPKIMEETH
WTVWITRFYIIGLFLFLTFLVFLAERRRNHESA
Number of residues
513
Molecular Weight
57407.51
Theoretical pI
9.17
GO Classification
Functions
neurotransmitter
Components
integral component of membrane
General Function
Neurotransmitter:sodium symporter activity
Specific Function
Not Available
Pfam Domain Function
Transmembrane Regions
12-29 41-61 92-111 166-185 197-215 243-268 289-314 342-367 379-399 405-427 447-469 481-504
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0012740|Transporter (snf)
ATGGAAGTTAAAAGGGAACACTGGGCGACGCGACTCGGTTTAATCCTCGCGATGGCGGGG
AACGCGGTAGGACTTGGTAATTTCCTCAGATTTCCCGTTCAAGCTGCGGAAAACGGTGGC
GGGGCTTTCATGATCCCATACATAATAGCCTTCCTCCTCGTGGGTATTCCTCTTATGTGG
ATAGAGTGGGCTATGGGAAGATACGGGGGAGCTCAGGGACACGGAACCACACCAGCTATT
TTTTACCTCCTCTGGAGAAACAGGTTTGCTAAAATTCTTGGAGTTTTCGGACTCTGGATA
CCTCTGGTGGTAGCCATATACTACGTTTACATAGAGAGCTGGACACTAGGTTTTGCGATA
AAGTTTTTGGTAGGGTTAGTTCCAGAACCTCCGCCAAACGCCACGGATCCCGATTCCATT
CTCAGACCCTTTAAAGAATTTCTGTACTCCTACATCGGAGTTCCTAAAGGAGATGAGCCG
ATTTTGAAACCTTCACTTTTTGCGTACATAGTCTTTTTAATAACTATGTTCATTAACGTT
TCCATTCTCATAAGGGGTATATCCAAGGGTATAGAGAGGTTTGCAAAAATAGCTATGCCC
ACGCTCTTTATCCTCGCGGTTTTTCTGGTCATCAGGGTCTTTTTACTGGAAACCCCCAAC
GGCACCGCGGCGGACGGACTTAACTTCCTTTGGACTCCCGACTTTGAGAAATTAAAGGAC
CCGGGCGTCTGGATAGCGGCTGTAGGACAAATATTCTTCACCCTGAGTCTTGGTTTTGGA
GCTATAATCACTTACGCCTCTTACGTGAGAAAGGATCAGGACATTGTCCTCAGCGGACTT
ACGGCGGCGACGTTGAACGAAAAGGCTGAGGTAATACTGGGTGGTTCTATTTCCATACCC
GCTGCGGTTGCTTTCTTCGGAGTTGCAAATGCGGTTGCGATTGCAAAGGCGGGAGCCTTT
AACCTCGGTTTTATAACCCTACCCGCCATATTCTCCCAAACGGCCGGAGGAACCTTTTTA
GGATTTCTGTGGTTCTTCCTCCTCTTCTTCGCAGGACTCACTTCTTCAATAGCTATAATG
CAACCGATGATAGCCTTCTTAGAGGACGAACTAAAGCTTAGCAGAAAACACGCTGTTCTC
TGGACCGCGGCTATAGTTTTCTTCAGTGCACACCTTGTGATGTTCTTAAACAAGTCCCTT
GACGAGATGGACTTCTGGGCGGGAACGATAGGGGTTGTTTTCTTTGGTCTCACAGAGCTC
ATAATCTTCTTCTGGATTTTCGGAGCGGATAAAGCTTGGGAGGAGATAAACAGGGGCGGA
ATTATTAAAGTTCCCAGAATTTATTATTACGTAATGAGGTATATAACCCCCGCTTTCCTC
GCGGTGCTCCTCGTTGTGTGGGCACGGGAGTACATACCGAAGATCATGGAAGAGACCCAC
TGGACCGTGTGGATAACACGCTTTTACATAATAGGGCTTTTCCTCTTCCTCACTTTCCTC
GTTTTCTTAGCTGAACGGAGAAGAAACCATGAGAGTGCTTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDO67854
UniProtKB Entry NameO67854_AQUAE
GenBank Protein ID2982776
GenBank Gene IDAE000657
General References
  1. Deckert G, Warren PV, Gaasterland T, Young WG, Lenox AL, Graham DE, Overbeek R, Snead MA, Keller M, Aujay M, Huber R, Feldman RA, Short JM, Olsen GJ, Swanson RV: The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature. 1998 Mar 26;392(6674):353-8. [PubMed:9537320]
  2. Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E: Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters. Nature. 2005 Sep 8;437(7056):215-23. Epub 2005 Jul 24. [PubMed:16041361]
  3. Singh SK, Yamashita A, Gouaux E: Antidepressant binding site in a bacterial homologue of neurotransmitter transporters. Nature. 2007 Aug 23;448(7156):952-6. Epub 2007 Aug 8. [PubMed:17687333]
  4. Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN: LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake. Science. 2007 Sep 7;317(5843):1390-3. Epub 2007 Aug 9. [PubMed:17690258]
  5. Singh SK, Piscitelli CL, Yamashita A, Gouaux E: A competitive inhibitor traps LeuT in an open-to-out conformation. Science. 2008 Dec 12;322(5908):1655-61. doi: 10.1126/science.1166777. [PubMed:19074341]
  6. Zhou Z, Zhen J, Karpowich NK, Law CJ, Reith ME, Wang DN: Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures. Nat Struct Mol Biol. 2009 Jun;16(6):652-7. doi: 10.1038/nsmb.1602. Epub 2009 May 10. [PubMed:19430461]
  7. Quick M, Winther AM, Shi L, Nissen P, Weinstein H, Javitch JA: Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5563-8. doi: 10.1073/pnas.0811322106. Epub 2009 Mar 23. [PubMed:19307590]
  8. Kroncke BM, Horanyi PS, Columbus L: Structural origins of nitroxide side chain dynamics on membrane protein alpha-helical sites. Biochemistry. 2010 Nov 30;49(47):10045-60. doi: 10.1021/bi101148w. Epub 2010 Nov 8. [PubMed:20964375]
  9. Piscitelli CL, Gouaux E: Insights into transport mechanism from LeuT engineered to transport tryptophan. EMBO J. 2012 Jan 4;31(1):228-35. doi: 10.1038/emboj.2011.353. Epub 2011 Sep 27. [PubMed:21952050]
  10. Wang H, Gouaux E: Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue. EMBO Rep. 2012 Sep;13(9):861-6. doi: 10.1038/embor.2012.110. Epub 2012 Jul 27. [PubMed:22836580]
  11. Wang H, Elferich J, Gouaux E: Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context. Nat Struct Mol Biol. 2012 Jan 15;19(2):212-9. doi: 10.1038/nsmb.2215. [PubMed:22245965]
  12. Krishnamurthy H, Gouaux E: X-ray structures of LeuT in substrate-free outward-open and apo inward-open states. Nature. 2012 Jan 9;481(7382):469-74. doi: 10.1038/nature10737. [PubMed:22230955]
  13. Wang H, Goehring A, Wang KH, Penmatsa A, Ressler R, Gouaux E: Structural basis for action by diverse antidepressants on biogenic amine transporters. Nature. 2013 Nov 7;503(7474):141-5. doi: 10.1038/nature12648. Epub 2013 Oct 13. [PubMed:24121440]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB08472(R)-FluoxetineexperimentalunknownDetails
DB08544(S)-FluoxetineexperimentalunknownDetails