Protocatechuate 3,4-dioxygenase beta chain

Details

Name

Protocatechuate 3,4-dioxygenase beta chain

Synonyms

• 1.13.11.3
• 3,4-PCD

Gene Name

pcaH

Organism

Pseudomonas putida

Amino acid sequence

>lcl|BSEQ0012811|Protocatechuate 3,4-dioxygenase beta chain
MPAQDNSRFVIRDRNWHPKALTPDYKTSIARSPRQALVSIPQSISETTGPNFSHLGFGAH
DHDLLLNFNNGGLPIGERIIVAGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPL
DPNFGGVGRCLTDSDGYYSFRTIKPGPYPWRNGPNDWRPAHIHFGISGPSIATKLITQLY
FEGDPLIPMCPIVKSIANPEAVQQLIAKLDMNNANPMDCLAYRFDIVLRGQRKTHFENC

Number of residues

239

Molecular Weight

26793.25

Theoretical pI

9.12

GO Classification

Functions

ferric iron binding / protocatechuate 3,4-dioxygenase activity

Processes

3,4-dihydroxybenzoate catabolic process / beta-ketoadipate pathway

General Function

Protocatechuate 3,4-dioxygenase activity

Specific Function

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Pfam Domain Function

• Dioxygenase_C (PF00775)
• PCDO_beta_N (PF12391)

Transmembrane Regions

Not Available

Cellular Location

Not Available

Gene sequence

>lcl|BSEQ0007713|720 bp
ATGCCCGCCCAGGACAACAGCCGCTTCGTGATCCGTGATCGCAACTGGCACCCCAAAGCC
CTTACGCCTGACTACAAAACGTCCATTGCCCGCTCGCCGCGCCAGGCACTGGTCAGCATT
CCACAGTCGATCAGCGAAACCACTGGTCCGAACTTTTCCCACCTGGGCTTCGGCGCCCAC
GACCATGACCTGCTGCTGAACTTCAACAACGGTGGCCTGCCAATCGGCGAGCGCATCATC
GTGGCCGGCCGCGTCGTCGACCAGTACGGCAAGCCTGTGCCGAACACCCTGGTGGAGATG
TGGCAAGCCAACGCCGGTGGCCGCTACCGGCACAAGAACGACCGTTACCTGGCACCGCTG
GACCCGAACTTTGGTGGTGTCGGCCGTTGCCTGACCGACAGCGACGGCTACTACAGCTTC
CGCACCATCAAGCCGGGCCCGTACCCCTGGCGCAACGGCCCGAACGACTGGCGCCCGGCG
CACATCCACTTCGGCATCAGCGGCCCGTCGATTGCGACCAAGCTGATCACCCAGTTGTAT
TTCGAGGGTGACCCGCTGATCCCGATGTGCCCGATCGTCAAGTCGATCGCCAACCCTGAA
GCTGTACAGCAGTTGATCGCCAAGCTCGACATGAACAACGCCAACCCGATGGACTGCCTG
GCCTACCGCTTTGACATCGTGCTGCGCGGCCAGCGCAAGACCCACTTCGAAAACTGCTGA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P00437
UniProtKB Entry Name	PCXB_PSEPU
GenBank Protein ID	294344
GenBank Gene ID	L14836

General References

1. Frazee RW, Livingston DM, LaPorte DC, Lipscomb JD: Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3,4-dioxygenase genes. J Bacteriol. 1993 Oct;175(19):6194-202. [Article]
2. Iwaki M, Kagamiyama H, Nozaki M: The complete amino acid sequence of the beta-subunit of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. J Biochem. 1979 Oct;86(4):1159-62. [Article]
3. Ohlendorf DH, Lipscomb JD, Weber PC: Structure and assembly of protocatechuate 3,4-dioxygenase. Nature. 1988 Nov 24;336(6197):403-5. [Article]
4. Ohlendorf DH, Orville AM, Lipscomb JD: Structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa at 2.15 A resolution. J Mol Biol. 1994 Dec 16;244(5):586-608. [Article]
5. Orville AM, Elango N, Lipscomb JD, Ohlendorf DH: Structures of competitive inhibitor complexes of protocatechuate 3,4-dioxygenase: multiple exogenous ligand binding orientations within the active site. Biochemistry. 1997 Aug 19;36(33):10039-51. [Article]
6. Orville AM, Lipscomb JD, Ohlendorf DH: Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding. Biochemistry. 1997 Aug 19;36(33):10052-66. [Article]
7. Elgren TE, Orville AM, Kelly KA, Lipscomb JD, Ohlendorf DH, Que L Jr: Crystal structure and resonance Raman studies of protocatechuate 3,4-dioxygenase complexed with 3,4-dihydroxyphenylacetate. Biochemistry. 1997 Sep 23;36(38):11504-13. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB01702	2-(3,4-Dihydroxyphenyl)Acetic Acid	experimental	unknown		Details