Lactose operon repressor

Details

Name
Lactose operon repressor
Synonyms
Not Available
Gene Name
lacI
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0005039|Lactose operon repressor
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQ
SLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVS
GLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQ
QIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPT
AMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTS
VDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
Number of residues
360
Molecular Weight
38589.815
Theoretical pI
6.88
GO Classification
Functions
bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding / transcriptional repressor activity, bacterial-type RNA polymerase core promoter proximal region sequence-specific binding
Processes
negative regulation of transcription, DNA-templated / transcription, DNA-templated
Components
cytosol
General Function
Transcriptional repressor activity, bacterial-type rna polymerase core promoter proximal region sequence-specific binding
Specific Function
Repressor of the lactose operon. Binds allolactose as an inducer.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Gene sequence
>lcl|BSEQ0020621|Lactose operon repressor (lacI)
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTT
TCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCG
GCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAG
TCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTC
GCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAA
CGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGT
GGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGC
ACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATT
TTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAG
CAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGC
TGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGG
AGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACT
GCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCC
GGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCA
TGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGC
GTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCC
GTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGC
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAG
TGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP03023
UniProtKB Entry NameLACI_ECOLI
GenBank Gene IDV00294
General References
  1. Farabaugh PJ: Sequence of the lacI gene. Nature. 1978 Aug 24;274(5673):765-9. [Article]
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  4. Beyreuther K, Adler K, Fanning E, Murray C, Klemm A, Geisler N: Amino-acid sequence of lac repressor from Escherichia coli. Isolation, sequence analysis and sequence assembly of tryptic peptides and cyanogen-bromide fragments. Eur J Biochem. 1975 Nov 15;59(2):491-509. [Article]
  5. Platt T, Files JG, Weber K: Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity. J Biol Chem. 1973 Jan 10;248(1):110-21. [Article]
  6. Ganem D, Miller JH, Files JG, Platt T, Weber K: Reinitiation of a lac repressor fragment at a codon other than AUG. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3165-9. [Article]
  7. Gordon AJ, Burns PA, Fix DF, Yatagai F, Allen FL, Horsfall MJ, Halliday JA, Gray J, Bernelot-Moens C, Glickman BW: Missense mutation in the lacI gene of Escherichia coli. Inferences on the structure of the repressor protein. J Mol Biol. 1988 Mar 20;200(2):239-51. [Article]
  8. Kamashev DE, Esipova NG, Ebralidse KK, Mirzabekov AD: Mechanism of Lac repressor switch-off: orientation of the Lac repressor DNA-binding domain is reversed upon inducer binding. FEBS Lett. 1995 Nov 13;375(1-2):27-30. [Article]
  9. Lehming N, Sartorius J, Kisters-Woike B, von Wilcken-Bergmann B, Muller-Hill B: Mutant lac repressors with new specificities hint at rules for protein--DNA recognition. EMBO J. 1990 Mar;9(3):615-21. [Article]
  10. Markiewicz P, Kleina LG, Cruz C, Ehret S, Miller JH: Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence. J Mol Biol. 1994 Jul 29;240(5):421-33. [Article]
  11. VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
  12. Kisters-Woike B, Lehming N, Sartorius J, von Wilcken-Bergmann B, Muller-Hill B: A model of the lac repressor-operator complex based on physical and genetic data. Eur J Biochem. 1991 Jun 1;198(2):411-9. [Article]
  13. Shin JA, Ebright RH, Dervan PB: Orientation of the Lac repressor DNA binding domain in complex with the left lac operator half site characterized by affinity cleaving. Nucleic Acids Res. 1991 Oct 11;19(19):5233-6. [Article]
  14. Boelens R, Lamerichs RM, Rullmann JA, van Boom JH, Kaptein R: The interaction of lac repressor headpiece with its operator: an NMR view. Protein Seq Data Anal. 1988;1(6):487-98. [Article]
  15. Lamerichs RM, Boelens R, van der Marel GA, van Boom JH, Kaptein R, Buck F, Fera B, Ruterjans H: H NMR study of a complex between the lac repressor headpiece and a 22 base pair symmetric lac operator. Biochemistry. 1989 Apr 4;28(7):2985-91. [Article]
  16. Slijper M, Bonvin AM, Boelens R, Kaptein R: Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator. J Mol Biol. 1996 Jun 21;259(4):761-73. [Article]
  17. Spronk CA, Bonvin AM, Radha PK, Melacini G, Boelens R, Kaptein R: The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator. Structure. 1999 Dec 15;7(12):1483-92. [Article]
  18. Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P: Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science. 1996 Mar 1;271(5253):1247-54. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB01862Isopropyl beta-D-thiogalactopyranosideexperimentalunknownDetails
DB08297ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDEexperimentalunknownDetails