Deoxynucleotide monophosphate kinase
Details
- Name
- Deoxynucleotide monophosphate kinase
- Synonyms
- 2.7.4.13
- DNK
- Gp1
- Gene Name
- 1
- Organism
- Enterobacteria phage T4
- Amino acid sequence
>lcl|BSEQ0016629|Deoxynucleotide monophosphate kinase MKLIFLSGVKRSGKDTTADFIMSNYSAVKYQLAGPIKDALAYAWGVFAANTDYPCLTRKE FEGIDYDRETNLNLTKLEVITIMEQAFCYLNGKSPIKGVFVFDDEGKESVNFVAFNKITD VINNIEDQWSVRRLMQALGTDLIVNNFDRMYWVKLFALDYLDKFNSGYDYYIVPDTRQDH EMDAARAMGATVIHVVRPGQKSNDTHITEAGLPIRDGDLVITNDGSLEELFSKIKNTLKV L
- Number of residues
- 241
- Molecular Weight
- 27328.9
- Theoretical pI
- 4.85
- GO Classification
- Functions(deoxy)nucleoside-phosphate kinase activity / ATP binding
- General Function
- Atp binding
- Specific Function
- Phosphorylates dGMP, dTMP and 5-hydroxymethyl-dCMP while excluding dCMP and dAMP. The phosphorylation of 5-hydroxymethyl-dCMP represents the first step in the replacement of cytosine by hydroxymethylcytosine in new viral DNA genomes.
- Pfam Domain Function
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016630|Deoxynucleotide monophosphate kinase (1) ATGAAACTAATCTTTTTAAGCGGTGTAAAGCGTAGTGGAAAAGATACTACTGCTGATTTT ATCATGAGCAATTATTCTGCAGTTAAATACCAACTTGCTGGTCCTATTAAGGATGCATTG GCTTATGCATGGGGAGTATTTGCAGCAAACACTGACTATCCTTGCTTAACTCGTAAAGAG TTTGAAGGAATTGACTATGATCGTGAGACTAATTTAAATCTGACTAAATTAGAAGTAATC ACGATTATGGAACAAGCATTTTGTTATCTTAATGGTAAAAGCCCAATTAAAGGTGTGTTT GTTTTTGATGACGAAGGAAAAGAATCAGTTAATTTCGTAGCATTTAATAAGATTACTGAC GTTATAAATAATATTGAAGATCAATGGTCAGTCCGTCGTCTGATGCAAGCCCTAGGTACG GATTTGATTGTTAATAACTTCGACCGCATGTACTGGGTAAAATTATTTGCTTTAGATTAT CTTGATAAATTTAACTCAGGTTATGATTATTATATCGTTCCTGATACCCGTCAAGATCAT GAAATGGATGCGGCTAGGGCGATGGGTGCTACAGTAATTCATGTAGTTCGTCCTGGTCAA AAATCCAATGATACACATATTACAGAAGCTGGATTGCCAATTCGTGATGGCGATTTAGTA ATTACAAACGATGGTTCTCTTGAAGAACTTTTTTCTAAAATTAAAAATACACTAAAGGTA CTATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P04531 UniProtKB Entry Name DNMK_BPT4 GenBank Protein ID 15387 GenBank Gene ID X03016 - General References
- Broida J, Abelson J: Sequence organization and control of transcription in the bacteriophage T4 tRNA region. J Mol Biol. 1985 Oct 5;185(3):545-63. [Article]
- Miller ES, Kutter E, Mosig G, Arisaka F, Kunisawa T, Ruger W: Bacteriophage T4 genome. Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. [Article]
- Herrmann R: Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence. Nucleic Acids Res. 1982 Feb 11;10(3):1105-12. [Article]
- Koch T, Lamm N, Ruger W: Sequencing, cloning and overexpression of genes of bacteriophage T4 between map positions 74.325 and 77.184. Nucleic Acids Res. 1989 Jun 12;17(11):4392. [Article]
- Duckworth DH, Bessman MJ: The enzymology of virus-infected bacteria. X. A biochemical-genetic study of the deoxynucleotide kinase induced by wild type and amber mutants of phage T4. J Biol Chem. 1967 Jun 25;242(12):2877-85. [Article]
- Teplyakov A, Sebastiao P, Obmolova G, Perrakis A, Brush GS, Bessman MJ, Wilson KS: Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP. EMBO J. 1996 Jul 15;15(14):3487-97. [Article]