Deoxyribodipyrimidine photo-lyase
Details
- Name
- Deoxyribodipyrimidine photo-lyase
- Synonyms
- 4.1.99.3
- DNA photolyase
- Photoreactivating enzyme
- phrA
- Gene Name
- phr
- Organism
- Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)
- Amino acid sequence
>lcl|BSEQ0016945|Deoxyribodipyrimidine photo-lyase MAAPILFWHRRDLRLSDNIGLAAARAQSAQLIGLFCLDPQILQSADMAPARVAYLQGCLQ ELQQRYQQAGSRLLLLQGDPQHLIPQLAQQLQAEAVYWNQDIEPYGRDRDGQVAAALKTA GIRAVQLWDQLLHSPDQILSGSGNPYSVYGPFWKNWQAQPKPTPVATPTELVDLSPEQLT AIAPLLLSELPTLKQLGFDWDGGFPVEPGETAAIARLQEFCDRAIADYDPQRNFPAEAGT SGLSPALKFGAIGIRQAWRAASAAHALSRSDEARNSIRVWQQELAWREFYQHALYHFPSL ADGPYRSLWQQFPWENREALFTAWTQAQTGYPIVDAAMRQLTETGWMHNRCWMIVASFLT KDLIIDWRRGEQFFMQHLVDGDLAANNGGWQWSASSGMDPKPLRIFNPASQAKKFDATAT YIKRWLPELRHVHPKDLISGEITPIGRRGYPAPIVNHNLRQKQFKALYNQLKAAIAEPEA EPDS
- Number of residues
- 484
- Molecular Weight
- 54461.235
- Theoretical pI
- 6.74
- GO Classification
- Functionsdeoxyribodipyrimidine photo-lyase activity / DNA binding / nucleotide bindingProcessesDNA repair / protein-chromophore linkage
- General Function
- Nucleotide binding
- Specific Function
- Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016946|Deoxyribodipyrimidine photo-lyase (phr) GTGGCGGCTCCGATTCTGTTTTGGCACCGGCGTGACCTGCGCCTCAGCGACAACATTGGT CTGGCGGCGGCGCGGGCACAATCAGCGCAGCTGATCGGTCTGTTTTGCCTCGACCCTCAG ATTCTCCAGAGTGCTGACATGGCGCCAGCACGGGTTGCCTACCTGCAGGGCTGCCTACAG GAACTCCAGCAGCGTTATCAACAGGCGGGCAGTCGGCTGCTATTGCTCCAAGGGGATCCA CAACACCTGATCCCGCAACTGGCGCAGCAGCTTCAGGCGGAAGCAGTTTATTGGAACCAA GACATTGAACCCTACGGCCGCGATCGCGATGGGCAGGTCGCAGCCGCTCTGAAAACGGCA GGCATTCGGGCTGTTCAACTCTGGGATCAACTGCTCCATAGCCCCGATCAAATCCTCAGC GGCAGCGGTAATCCCTACAGCGTCTACGGGCCGTTTTGGAAAAATTGGCAGGCTCAGCCC AAGCCCACGCCGGTTGCCACACCGACAGAACTGGTCGATCTCTCACCAGAGCAACTAACT GCGATCGCACCTCTGCTGTTGTCGGAACTACCGACCCTGAAGCAGCTTGGCTTTGACTGG GACGGGGGTTTTCCAGTGGAGCCGGGGGAAACAGCGGCGATCGCCCGTCTTCAAGAATTT TGCGATCGCGCGATCGCCGACTACGATCCCCAGCGCAATTTTCCGGCGGAAGCAGGAACC TCGGGCTTGAGTCCCGCTTTGAAATTCGGGGCGATCGGTATTCGTCAGGCTTGGCGGGCT GCCAGTGCAGCCCATGCCCTCAGCCGCAGCGACGAAGCCCGCAACAGCATTCGCGTTTGG CAGCAGGAACTCGCTTGGCGGGAGTTCTATCAGCATGCGCTCTACCATTTCCCGAGCCTG GCCGATGGTCCCTATCGCTCGCTCTGGCAGCAATTCCCTTGGGAAAATCGCGAGGCTCTG TTCACGGCTTGGACCCAGGCGCAAACGGGCTATCCGATTGTTGATGCAGCGATGCGCCAA CTGACTGAAACCGGCTGGATGCACAACCGTTGCTGGATGATTGTTGCCAGCTTTTTGACC AAGGATTTGATCATCGACTGGCGGCGTGGGGAGCAGTTCTTTATGCAGCACTTGGTCGAT GGCGATCTGGCTGCAAATAACGGCGGTTGGCAGTGGAGCGCTTCCAGTGGCATGGATCCC AAACCGCTGCGGATTTTCAATCCTGCTAGTCAGGCCAAGAAGTTTGATGCCACGGCGACC TACATCAAACGTTGGCTGCCAGAACTGCGCCATGTCCATCCCAAGGACTTGATCAGTGGG GAGATCACGCCGATTGGACGCCGAGGCTATCCTGCCCCGATCGTGAATCACAACCTGCGC CAGAAACAGTTCAAGGCGCTCTACAACCAGCTCAAAGCGGCTATCGCTGAACCTGAGGCT GAGCCCGATTCATAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P05327 UniProtKB Entry Name PHR_SYNP6 GenBank Gene ID X07230 - General References
- Yasui A, Takao M, Oikawa A, Kiener A, Walsh CT, Eker AP: Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans. Nucleic Acids Res. 1988 May 25;16(10):4447-63. [Article]
- Sugita C, Ogata K, Shikata M, Jikuya H, Takano J, Furumichi M, Kanehisa M, Omata T, Sugiura M, Sugita M: Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization. Photosynth Res. 2007 Jul-Sep;93(1-3):55-67. Epub 2007 Jan 9. [Article]
- Eker AP, Kooiman P, Hessels JK, Yasui A: DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans. J Biol Chem. 1990 May 15;265(14):8009-15. [Article]
- Weber S: Light-driven enzymatic catalysis of DNA repair: a review of recent biophysical studies on photolyase. Biochim Biophys Acta. 2005 Feb 25;1707(1):1-23. [Article]
- Tamada T, Kitadokoro K, Higuchi Y, Inaka K, Yasui A, de Ruiter PE, Eker AP, Miki K: Crystal structure of DNA photolyase from Anacystis nidulans. Nat Struct Biol. 1997 Nov;4(11):887-91. [Article]
- Kort R, Komori H, Adachi S, Miki K, Eker A: DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction. Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1205-13. Epub 2004 Jun 22. [Article]
- Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO: Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair. Science. 2004 Dec 3;306(5702):1789-93. [Article]