Neocarzinostatin

Details

Name
Neocarzinostatin
Synonyms
  • Mitomalcin
  • MMC
  • NCS
Gene Name
ncsA
Organism
Streptomyces carzinostaticus
Amino acid sequence
>lcl|BSEQ0017324|Neocarzinostatin
MVPISIIRNRVAKVAVGSAAVLGLAVGFQTPAVAAAPTATVTPSSGLSDGTVVKVAGAGL
QAGTAYDVGQCAWVDTGVLACNPADFSSVTADANGSASTSLTVRRSFEGFLFDGTRWGTV
DCTTAACQVGLSDAAGNGPEGVAISFN
Number of residues
147
Molecular Weight
14455.08
Theoretical pI
4.34
GO Classification
Functions
DNA binding
Processes
defense response to bacterium
General Function
NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
Specific Function
Dna binding
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Gene sequence
>lcl|BSEQ0007788|444 bp
GTGGTCCCCATTTCCATCATCAGGAATCGAGTAGCGAAGGTCGCCGTCGGCTCGGCGGCG
GTGCTGGGGCTCGCCGTAGGGTTCCAGACCCCGGCCGTGGCCGCGGCGCCGACGGCTACG
GTGACTCCGTCGTCCGGTCTGTCCGACGGCACCGTGGTCAAGGTCGCCGGCGCGGGTCTC
CAGGCCGGAACGGCCTACGACGTCGGGCAGTGCGCGTGGGTGGACACCGGTGTTCTCGCG
TGCAACCCGGCGGACTTCTCCTCCGTGACCGCGGACGCCAACGGCTCCGCGAGCACGTCG
CTGACGGTGCGCCGCTCCTTCGAGGGCTTCCTCTTCGACGGCACCCGCTGGGGCACCGTG
GACTGCACCACCGCGGCCTGCCAGGTCGGCCTCTCGGACGCTGCGGGCAACGGCCCGGAG
GGTGTGGCGATCTCCTTCAACTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0A3R9
UniProtKB Entry NameNCZS_STRCZ
GenBank Protein ID398596
GenBank Gene IDD10996
General References
  1. Sakata N, Minamitani S, Kanbe T, Hori M, Hamada M, Edo K: The amino acid sequence of neocarzinostatin apoprotein deduced from the base sequence of the gene. Biol Pharm Bull. 1993 Jan;16(1):26-8. [Article]
  2. Kuromizu K, Tsunasawa S, Maeda H, Abe O, Sakiyama F: Reexamination of the primary structure of an antitumor protein, neocarzinostatin. Arch Biochem Biophys. 1986 Apr;246(1):199-205. [Article]
  3. Gibson BW, Herlihy WC, Samy TS, Hahm KS, Maeda H, Meienhofer J, Biemann K: A revised primary structure for neocarzinostatin based on fast atom bombardment and gas chromatographic-mass spectrometry. J Biol Chem. 1984 Sep 10;259(17):10801-6. [Article]
  4. Gao XL, Burkhart W: Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Biochemistry. 1991 Aug 6;30(31):7730-9. [Article]
  5. Teplyakov A, Obmolova G, Wilson K, Kuromizu K: Crystal structure of apo-neocarzinostatin at 0.15-nm resolution. Eur J Biochem. 1993 Apr 15;213(2):737-41. [Article]
  6. Kim KH, Kwon BM, Myers AG, Rees DC: Crystal structure of neocarzinostatin, an antitumor protein-chromophore complex. Science. 1993 Nov 12;262(5136):1042-6. [Article]
  7. Gao X: Three-dimensional solution structure of apo-neocarzinostatin. J Mol Biol. 1992 May 5;225(1):125-35. [Article]
  8. Adjadj E, Mispelter J, Quiniou E, Dimicoli JL, Favaudon V, Lhoste JM: Proton NMR studies of apo-neocarzinostatin from Streptomyces carzinostaticus. Sequence-specific assignment and secondary structure. Eur J Biochem. 1990 Jun 20;190(2):263-71. [Article]
  9. Adjadj E, Quiniou E, Mispelter J, Favaudon V, Lhoste JM: Three-dimensional solution structure of apo-neocarzinostatin from Streptomyces carzinostaticus determined by NMR spectroscopy. Eur J Biochem. 1992 Feb 1;203(3):505-11. [Article]
  10. Remerowski ML, Glaser SJ, Sieker LC, Samy TS, Drobny GP: Sequential 1H NMR assignments and secondary structure of aponeocarzinostatin in solution. Biochemistry. 1990 Sep 11;29(36):8401-9. [Article]
  11. Takashima H, Amiya S, Kobayashi Y: Neocarzinostatin: interaction between the antitumor-active chromophore and the carrier protein. J Biochem. 1991 Jun;109(6):807-10. [Article]
  12. Kuromizu K, Abe O, Maeda H: Location of the disulfide bonds in the antitumor protein neocarzinostatin. Arch Biochem Biophys. 1991 May 1;286(2):569-73. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB07776Flavoneapproved, experimentalunknownDetails
DB082612-hydroxy-7-methoxy-5-methyl-naphthalene-1-carboxylic acid meso-2,5-dihydroxy-cyclopent-3-enyl esterexperimentalunknownDetails
DB08619Testosterone succinateexperimentalunknownDetails