Aspartate ammonia-lyase

Details

Name

Aspartate ammonia-lyase

Synonyms

• 4.3.1.1
• Aspartase

Gene Name

aspA

Organism

Escherichia coli (strain K12)

Amino acid sequence

>lcl|BSEQ0004076|Aspartate ammonia-lyase
MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAM
ANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLE
LMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSLIKLVDAINQLREGFERKAVE
FQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNIQRTAELLLEVNLGATAIGTGLN
TPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGAYVMVHGALKRLAVKMSKICNDLRL
LSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTMAAEAGQLQ
LNVMEPVIGQAMFESVHILTNACYNLLEKCINGITANKEVCEGYVYNSIGIVTYLNPFIG
HHNGDIVGKICAETGKSVREVVLERGLLTEAELDDIFSVQNLMHPAYKAKRYTDESEQ

Number of residues

478

Molecular Weight

52355.755

Theoretical pI

4.96

GO Classification

Functions

aspartate ammonia-lyase activity

Processes

aspartate metabolic process / cellular amino acid biosynthetic process / protein tetramerization

Components

cytosol / membrane

General Function

Aspartate ammonia-lyase activity

Specific Function

Not Available

Pfam Domain Function

• Lyase_1 (PF00206)
• FumaraseC_C (PF10415)

Transmembrane Regions

Not Available

Cellular Location

Not Available

Gene sequence

>lcl|BSEQ0019232|Aspartate ammonia-lyase (aspA)
ATGTCAAACAACATTCGTATCGAAGAAGATCTGTTGGGTACCAGGGAAGTTCCAGCTGAT
GCCTACTATGGTGTTCACACTCTGAGAGCGATTGAAAACTTCTATATCAGCAACAACAAA
ATCAGTGATATTCCTGAATTTGTTCGCGGTATGGTAATGGTTAAAAAAGCCGCAGCTATG
GCAAACAAAGAGCTGCAAACCATTCCTAAAAGTGTAGCGAATGCCATCATTGCCGCATGT
GATGAAGTCCTGAACAACGGAAAATGCATGGATCAGTTCCCGGTAGACGTCTACCAGGGC
GGCGCAGGTACTTCCGTAAACATGAACACCAACGAAGTGCTGGCCAATATCGGTCTGGAA
CTGATGGGTCACCAAAAAGGTGAATATCAGTACCTGAACCCGAACGACCATGTTAACAAA
TGTCAGTCCACTAACGACGCCTACCCGACCGGTTTCCGTATCGCAGTTTACTCTTCCCTG
ATTAAGCTGGTAGATGCGATTAACCAACTGCGTGAAGGCTTTGAACGTAAAGCTGTCGAA
TTCCAGGACATCCTGAAAATGGGTCGTACCCAGCTGCAGGACGCAGTACCGATGACCCTC
GGTCAGGAATTCCGCGCTTTCAGCATCCTGCTGAAAGAAGAAGTGAAAAACATCCAACGT
ACCGCTGAACTGCTGCTGGAAGTTAACCTTGGTGCAACAGCAATCGGTACTGGTCTGAAC
ACGCCGAAAGAGTACTCTCCGCTGGCAGTGAAAAAACTGGCTGAAGTTACTGGCTTCCCA
TGCGTACCGGCTGAAGACCTGATCGAAGCGACCTCTGACTGCGGCGCTTATGTTATGGTT
CACGGCGCGCTGAAACGCCTGGCTGTGAAGATGTCCAAAATCTGTAACGACCTGCGCTTG
CTCTCTTCAGGCCCACGTGCCGGCCTGAACGAGATCAACCTGCCGGAACTGCAGGCGGGC
TCTTCCATCATGCCAGCTAAAGTAAACCCGGTTGTTCCGGAAGTGGTTAACCAGGTATGC
TTCAAAGTCATCGGTAACGACACCACTGTTACCATGGCAGCAGAAGCAGGTCAGCTGCAG
TTGAACGTTATGGAGCCGGTCATTGGCCAGGCCATGTTCGAATCCGTTCACATTCTGACC
AACGCTTGCTACAACCTGCTGGAAAAATGCATTAACGGCATCACTGCTAACAAAGAAGTG
TGCGAAGGTTACGTTTACAACTCTATCGGTATCGTTACTTACCTGAACCCGTTCATCGGT
CACCACAACGGTGACATCGTGGGTAAAATCTGTGCCGAAACCGGTAAGAGTGTACGTGAA
GTCGTTCTGGAACGCGGTCTGTTGACTGAAGCGGAACTTGACGATATTTTCTCCGTACAG
AATCTGATGCACCCGGCTTACAAAGCAAAACGCTATACTGATGAAAGCGAACAGTAA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P0AC38
UniProtKB Entry Name	ASPA_ECOLI
GenBank Gene ID	X02307

General References

1. Takagi JS, Ida N, Tokushige M, Sakamoto H, Shimura Y: Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W. Nucleic Acids Res. 1985 Mar 25;13(6):2063-74. [Article]
2. Woods SA, Miles JS, Roberts RE, Guest JR: Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. Biochem J. 1986 Jul 15;237(2):547-57. [Article]
3. Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. Nucleic Acids Res. 1995 Jun 25;23(12):2105-19. [Article]
4. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
5. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
6. Saribas AS, Schindler JF, Viola RE: Mutagenic investigation of conserved functional amino acids in Escherichia coli L-aspartase. J Biol Chem. 1994 Mar 4;269(9):6313-9. [Article]
7. Jayasekera MM, Shi W, Farber GK, Viola RE: Evaluation of functionally important amino acids in L-aspartate ammonia-lyase from Escherichia coli. Biochemistry. 1997 Jul 29;36(30):9145-50. [Article]
8. Shi W, Dunbar J, Jayasekera MM, Viola RE, Farber GK: The structure of L-aspartate ammonia-lyase from Escherichia coli. Biochemistry. 1997 Jul 29;36(30):9136-44. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB02379	Beta-D-Glucose	experimental	unknown		Details