Osmolarity sensor protein EnvZ

Details

Name
Osmolarity sensor protein EnvZ
Synonyms
  • 2.7.13.3
  • ompB
  • perA
  • tpo
Gene Name
envZ
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016672|Osmolarity sensor protein EnvZ
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSS
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
Number of residues
450
Molecular Weight
50333.625
Theoretical pI
6.81
GO Classification
Functions
ATP binding / phosphoprotein phosphatase activity / phosphorelay sensor kinase activity
Processes
detection of temperature stimulus / peptidyl-histidine phosphorylation / phosphorelay signal transduction system / phosphorylation / protein autophosphorylation / protein dephosphorylation / regulation of response to osmotic stress / signal transduction by protein phosphorylation
Components
cytoplasmic side of plasma membrane / integral component of plasma membrane / intracellular
General Function
Phosphorelay sensor kinase activity
Specific Function
Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals.
Pfam Domain Function
Transmembrane Regions
16-35 159-179
Cellular Location
Cell inner membrane
Gene sequence
>lcl|BSEQ0016673|Osmolarity sensor protein EnvZ (envZ)
ATGAGGCGATTGCGCTTCTCGCCACGAAGTTCATTTGCCCGTACGTTATTGCTCATCGTC
ACCTTGCTGTTCGCCAGCCTGGTGACGACTTATCTGGTGGTGCTGAACTTCGCGATTTTG
CCGAGCCTCCAGCAGTTTAATAAAGTCCTCGCGTACGAAGTGCGTATGTTGATGACCGAC
AAACTGCAACTGGAGGACGGCACGCAGTTGGTTGTGCCTCCCGCTTTCCGTCGGGAGATC
TACCGTGAGCTGGGGATCTCTCTCTACTCCAACGAGGCTGCCGAAGAGGCAGGTCTGCGT
TGGGCGCAACACTATGAATTCTTAAGCCATCAGATGGCGCAGCAACTGGGCGGCCCGACG
GAAGTGCGCGTTGAGGTCAACAAAAGTTCGCCTGTCGTCTGGCTGAAAACCTGGCTGTCG
CCCAATATCTGGGTACGCGTGCCGCTGACCGAAATTCATCAGGGCGATTTCTCTCCGCTG
TTCCGCTATACGCTGGCGATTATGCTATTGGCGATAGGCGGGGCGTGGCTGTTTATTCGT
ATCCAGAACCGACCGTTGGTCGATCTCGAACACGCAGCCTTGCAGGTTGGTAAAGGGATT
ATTCCGCCGCCGCTGCGTGAGTATGGCGCTTCGGAGGTGCGTTCCGTTACCCGTGCCTTT
AACCATATGGCGGCTGGTGTTAAGCAACTGGCGGATGACCGCACGCTGCTGATGGCGGGG
GTAAGTCACGACTTGCGCACGCCGCTGACGCGTATTCGCCTGGCGACTGAGATGATGAGC
GAGCAGGATGGCTATCTGGCAGAATCGATCAATAAAGATATCGAAGAGTGCAACGCCATC
ATTGAGCAGTTTATCGACTACCTGCGCACCGGGCAGGAGATGCCGATGGAAATGGCGGAT
CTTAATGCAGTACTCGGTGAGGTGATTGCTGCCGAAAGTGGCTATGAGCGGGAAATTGAA
ACCGCGCTTTACCCCGGCAGCATTGAAGTGAAAATGCACCCGCTGTCGATCAAACGCGCG
GTGGCGAATATGGTGGTCAACGCCGCCCGTTATGGCAATGGCTGGATCAAAGTCAGCAGC
GGAACGGAGCCGAATCGCGCCTGGTTCCAGGTGGAAGATGACGGTCCGGGAATTGCGCCG
GAACAACGTAAGCACCTGTTCCAGCCGTTTGTCCGCGGCGACAGTGCGCGCACCATTAGC
GGCACGGGATTAGGGCTGGCAATTGTGCAGCGTATCGTGGATAACCATAACGGGATGCTG
GAGCTTGGCACCAGCGAGCGGGGCGGGCTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTA
ACGCGGGCGCAGGGCACGACAAAAGAAGGGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0AEJ4
UniProtKB Entry NameENVZ_ECOLI
GenBank Gene IDU18997
General References
  1. Mizuno T, Wurtzel ET, Inouye M: Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product. J Biol Chem. 1982 Nov 25;257(22):13692-8. [Article]
  2. Comeau DE, Ikenaka K, Tsung KL, Inouye M: Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol. 1985 Nov;164(2):578-84. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Roberts DL, Bennett DW, Forst SA: Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli. J Biol Chem. 1994 Mar 25;269(12):8728-33. [Article]
  6. Kanamaru K, Aiba H, Mizuno T: Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR. J Biochem. 1990 Sep;108(3):483-7. [Article]
  7. Forst S, Comeau D, Norioka S, Inouye M: Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli. J Biol Chem. 1987 Dec 5;262(34):16433-8. [Article]
  8. Tokishita S, Kojima A, Mizuno T: Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-located protein kinase, EnvZ. J Biochem. 1992 Jun;111(6):707-13. [Article]
  9. Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [Article]
  10. Khorchid A, Inouye M, Ikura M: Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization. Biochem J. 2005 Jan 1;385(Pt 1):255-64. [Article]
  11. Kishii R, Falzon L, Yoshida T, Kobayashi H, Inouye M: Structural and functional studies of the HAMP domain of EnvZ, an osmosensing transmembrane histidine kinase in Escherichia coli. J Biol Chem. 2007 Sep 7;282(36):26401-8. Epub 2007 Jul 16. [Article]
  12. Gerken H, Charlson ES, Cicirelli EM, Kenney LJ, Misra R: MzrA: a novel modulator of the EnvZ/OmpR two-component regulon. Mol Microbiol. 2009 Jun;72(6):1408-22. doi: 10.1111/j.1365-2958.2009.06728.x. Epub 2009 May 8. [Article]
  13. Gerken H, Misra R: MzrA-EnvZ interactions in the periplasm influence the EnvZ/OmpR two-component regulon. J Bacteriol. 2010 Dec;192(23):6271-8. doi: 10.1128/JB.00855-10. Epub 2010 Oct 1. [Article]
  14. Tanaka T, Saha SK, Tomomori C, Ishima R, Liu D, Tong KI, Park H, Dutta R, Qin L, Swindells MB, Yamazaki T, Ono AM, Kainosho M, Inouye M, Ikura M: NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Nature. 1998 Nov 5;396(6706):88-92. [Article]
  15. Tomomori C, Tanaka T, Dutta R, Park H, Saha SK, Zhu Y, Ishima R, Liu D, Tong KI, Kurokawa H, Qian H, Inouye M, Ikura M: Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ. Nat Struct Biol. 1999 Aug;6(8):729-34. [Article]
  16. Cai SJ, Khorchid A, Ikura M, Inouye M: Probing catalytically essential domain orientation in histidine kinase EnvZ by targeted disulfide crosslinking. J Mol Biol. 2003 Apr 25;328(2):409-18. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB04395Phosphoaminophosphonic Acid-Adenylate EsterexperimentalunknownDetails