Cellulose 1,4-beta-cellobiosidase (reducing end) CelS
Details
- Name
- Cellulose 1,4-beta-cellobiosidase (reducing end) CelS
- Synonyms
- 3.2.1.176
- Cellobiohydrolase CelS
- Cellulase SS
- EGSS
- Endo-1,4-beta-glucanase
- Endoglucanase SS
- Exocellulase
- Gene Name
- celS
- Organism
- Clostridium thermocellum
- Amino acid sequence
>lcl|BSEQ0016661|Cellulose 1,4-beta-cellobiosidase (reducing end) CelS MVKSRKISILLAVAMLVSIMIPTTAFAGPTKAPTKDGTSYKDLFLELYGKIKDPKNGYFS PDEGIPYHSIETLIVEAPDYGHVTTSEAFSYYVWLEAMYGNLTGNWSGVETAWKVMEDWI IPDSTEQPGMSSYNPNSPATYADEYEDPSYYPSELKFDTVRVGSDPVHNDLVSAYGPNMY LMHWLMDVDNWYGFGTGTRATFINTFQRGEQESTWETIPHPSIEEFKYGGPNGFLDLFTK DRSYAKQWRYTNAPDAEGRAIQAVYWANKWAKEQGKGSAVASVVSKAAKMGDFLRNDMFD KYFMKIGAQDKTPATGYDSAHYLMAWYTAWGGGIGASWAWKIGCSHAHFGYQNPFQGWVS ATQSDFAPKSSNGKRDWTTSYKRQLEFYQWLQSAEGGIAGGATNSWNGRYEKYPAGTSTF YGMAYVPHPVYADPGSNQWFGFQAWSMQRVMEYYLETGDSSVKNLIKKWVDWVMSEIKLY DDGTFAIPSDLEWSGQPDTWTGTYTGNPNLHVRVTSYGTDLGVAGSLANALATYAAATER WEGKLDTKARDMAAELVNRAWYNFYCSEGKGVVTEEARADYKRFFEQEVYVPAGWSGTMP NGDKIQPGIKFIDIRTKYRQDPYYDIVYQAYLRGEAPVLNYHRFWHEVDLAVAMGVLATY FPDMTYKVPGTPSTKLYGDVNDDGKVNSTDAVALKRYVLRSGISINTDNADLNEDGRVNS TDLGILKRYILKEIDTLPYKN
- Number of residues
- 741
- Molecular Weight
- 83557.84
- Theoretical pI
- 5.1
- GO Classification
- Functionscellulase activity / metal ion bindingProcessescellulose catabolic processComponentsextracellular region
- General Function
- Metal ion binding
- Specific Function
- This enzyme catalyzes the exohydrolysis of 1,4-beta-glucosidic linkages in cellulose with a preference for amorphous or crystalline cellulose over carboxymethyl cellulose.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0C2S5 UniProtKB Entry Name GUNS_CLOTM - General References
- Choi SK, Ljungdahl LG: Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of trucated polypeptides. Biochemistry. 1996 Apr 16;35(15):4897-905. [Article]
- Lytle BL, Volkman BF, Westler WM, Heckman MP, Wu JH: Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain. J Mol Biol. 2001 Mar 30;307(3):745-53. [Article]
- Saharay M, Guo H, Smith JC: Catalytic mechanism of cellulose degradation by a cellobiohydrolase, CelS. PLoS One. 2010 Oct 12;5(10):e12947. doi: 10.1371/journal.pone.0012947. [Article]
- Guimaraes BG, Souchon H, Lytle BL, David Wu JH, Alzari PM: The crystal structure and catalytic mechanism of cellobiohydrolase CelS, the major enzymatic component of the Clostridium thermocellum Cellulosome. J Mol Biol. 2002 Jul 12;320(3):587-96. [Article]