Respiratory nitrate reductase 1 beta chain
Details
- Name
- Respiratory nitrate reductase 1 beta chain
- Synonyms
- 1.7.99.4
- Nitrate reductase A subunit beta
- Quinol-nitrate oxidoreductase subunit beta
- Gene Name
- narH
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0012660|Respiratory nitrate reductase 1 beta chain MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGQGFPTDWENQE KYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYEPFDFDYQNLHTAPEGSKS QPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKNFDNIQKAMYSQFENTFMMYL PRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCRGWRMCITGCPYKKIYFNWKSGK SEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADAIERAASTENEKDLYQRQLDVF LDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMAMEWKLALPLHPEYRTLPMVWYVPPLS PIQSAADAGELGSNGILPDVESLRIPVQYLANLLTAGDTKPVLRALKRMLAMRHYKRAET VDGKVDTRALEEVGLTEAQAQEMYRYLAIANYEDRFVVPSSHRELAREAFPEKNGCGFTF GDGCHGSDTKFNLFNSRRIDAIDVTSKTEPHP
- Number of residues
- 512
- Molecular Weight
- 58065.915
- Theoretical pI
- 6.76
- GO Classification
- Functions3 iron, 4 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / electron carrier activity / metal ion binding / nitrate reductase activity / oxidoreductase activityProcessesanaerobic respiration / nitrate assimilation / nitrate metabolic processComponentscytoplasm / intrinsic component of membrane / intrinsic component of the cytoplasmic side of the plasma membrane / membrane / NarGHI complex
- General Function
- Oxidoreductase activity
- Specific Function
- The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell membrane
- Gene sequence
>lcl|BSEQ0012661|Respiratory nitrate reductase 1 beta chain (narH) ATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCAC ACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGG TTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGGGAAAACCAGGAA AAATACAAAGGCGGCTGGATCCGTAAAATCAACGGCAAACTGCAGCCGCGCATGGGTAAC CGTGCCATGCTGCTGGGTAAAATCTTCGCTAACCCGCATCTGCCGGGGATCGACGATTAT TACGAGCCGTTCGATTTTGACTATCAGAACCTGCATACCGCGCCGGAAGGCAGCAAATCG CAGCCGATTGCCCGTCCGCGTTCGCTGATTACCGGGGAACGGATGGCGAAAATCGAAAAA GGGCCGAACTGGGAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAAC TTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTG CCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCG ATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGG CGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACTTCAACTGGAAGAGCGGTAAG TCTGAGAAGTGCATCTTCTGCTATCCGCGTATTGAAGCGGGTCAGCCGACCGTGTGCTCA GAAACCTGTGTCGGTCGTATCCGTTATCTTGGCGTGCTGTTGTACGATGCCGACGCTATT GAACGTGCAGCCAGCACCGAGAACGAGAAAGATCTTTACCAGCGTCAGCTGGACGTGTTC CTCGATCCGAACGATCCGAAAGTCATCGAGCAGGCGATTAAAGACGGTATTCCGCTGAGC GTTATTGAAGCCGCACAGCAGTCGCCGGTTTATAAAATGGCAATGGAATGGAAACTGGCG CTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCT CCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTC GAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAA CCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACC GTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCA CAGGAGATGTACCGTTATCTGGCGATTGCTAACTACGAAGATCGCTTTGTGGTGCCGAGT AGTCATCGTGAACTGGCACGGGAAGCCTTCCCGGAGAAAAATGGCTGCGGCTTTACCTTT GGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGAT GCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11349 UniProtKB Entry Name NARH_ECOLI GenBank Protein ID 146919 GenBank Gene ID M20147 - General References
- Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V: Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer. Mol Gen Genet. 1989 Aug;218(2):249-56. [Article]
- Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Sodergren EJ, DeMoss JA: narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes. J Bacteriol. 1988 Apr;170(4):1721-9. [Article]
- Sodergren EJ, Hsu PY, DeMoss JA: Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli. J Biol Chem. 1988 Nov 5;263(31):16156-62. [Article]
- Guigliarelli B, Asso M, More C, Augier V, Blasco F, Pommier J, Giordano G, Bertrand P: EPR and redox characterization of iron-sulfur centers in nitrate reductases A and Z from Escherichia coli. Evidence for a high-potential and a low-potential class and their relevance in the electron-transfer mechanism. Eur J Biochem. 1992 Jul 1;207(1):61-8. [Article]
- Augier V, Guigliarelli B, Asso M, Bertrand P, Frixon C, Giordano G, Chippaux M, Blasco F: Site-directed mutagenesis of conserved cysteine residues within the beta subunit of Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of the mutated enzymes. Biochemistry. 1993 Mar 2;32(8):2013-23. [Article]
- Augier V, Asso M, Guigliarelli B, More C, Bertrand P, Santini CL, Blasco F, Chippaux M, Giordano G: Removal of the high-potential [4Fe-4S] center of the beta-subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutated enzymes. Biochemistry. 1993 May 18;32(19):5099-108. [Article]
- Guigliarelli B, Magalon A, Asso M, Bertrand P, Frixon C, Giordano G, Blasco F: Complete coordination of the four Fe-S centers of the beta subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutants lacking the highest or lowest potential [4Fe-4S] clusters. Biochemistry. 1996 Apr 16;35(15):4828-36. [Article]
- Rothery RA, Magalon A, Giordano G, Guigliarelli B, Blasco F, Weiner JH: The molybdenum cofactor of Escherichia coli nitrate reductase A (NarGHI). Effect of a mobAB mutation and interactions with [Fe-S] clusters. J Biol Chem. 1998 Mar 27;273(13):7462-9. [Article]
- Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC: Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A. Nat Struct Biol. 2003 Sep;10(9):681-7. Epub 2003 Aug 10. [Article]
- Jormakka M, Richardson D, Byrne B, Iwata S: Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes. Structure. 2004 Jan;12(1):95-104. [Article]