DNA topoisomerase 4 subunit B
Details
- Name
- DNA topoisomerase 4 subunit B
- Synonyms
- 5.99.1.3
- nfxD
- Topoisomerase IV subunit B
- Gene Name
- parE
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0006425|DNA topoisomerase 4 subunit B MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRFS VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQ NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALT EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD AMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
- Number of residues
- 630
- Molecular Weight
- 70243.365
- Theoretical pI
- 5.36
- GO Classification
- FunctionsATP binding / DNA binding / DNA topoisomerase type II (ATP-hydrolyzing) activity / magnesium ion bindingProcesseschromosome organization / DNA topological change / plasmid partitioning / response to antibiotic / sister chromatid cohesionComponentschromosome / cytosol
- General Function
- Magnesium ion binding
- Specific Function
- Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0020684|DNA topoisomerase 4 subunit B (parE) ATGACGCAAACTTATAACGCTGATGCCATTGAGGTACTCACCGGGCTTGAGCCGGTTCGC CGCCGTCCGGGGATGTATACCGATACCACTCGCCCTAACCATTTGGGGCAAGAAGTCATT GATAACAGTGTGGATGAAGCACTGGCGGGTCACGCAAAACGCGTGGACGTTATTTTACAT GCTGACCAGTCGTTAGAAGTTATTGACGATGGGCGCGGGATGCCGGTGGATATTCACCCG GAAGAGGGTGTACCGGCGGTTGAACTGATTCTTTGCCGTCTGCATGCAGGCGGTAAATTC TCTAACAAAAATTACCAGTTCTCTGGCGGCCTGCATGGCGTGGGGATTTCGGTGGTTAAC GCCCTGTCGAAGCGCGTAGAAGTTAACGTGCGCCGCGATGGTCAGGTTTATAACATCGCC TTTGAAAATGGCGAAAAGGTGCAGGATTTACAGGTTGTCGGCACTTGCGGTAAACGCAAT ACTGGTACCAGTGTGCACTTCTGGCCGGATGAAACCTTCTTTGACAGCCCGCGATTTTCT GTTTCACGCCTGACGCATGTGCTGAAAGCCAAAGCGGTATTGTGCCCTGGCGTTGAGATC ACTTTTAAAGATGAGATCAACAATACCGAACAACGCTGGTGCTATCAGGACGGTCTGAAT GATTACCTGGCGGAAGCGGTAAATGGTCTGCCGACGCTGCCGGAAAAACCGTTTATCGGT AATTTCGCTGGTGATACTGAAGCTGTGGACTGGGCGCTACTGTGGCTGCCGGAAGGCGGT GAACTGCTGACCGAAAGCTACGTCAACCTTATCCCAACGATGCAGGGCGGTACCCATGTT AATGGTCTGCGTCAGGGCCTGTTGGACGCGATGCGTGAGTTCTGTGAATACCGCAATATT CTGCCGCGCGGTGTAAAGCTGTCGGCGGAAGATATCTGGGATCGCTGCGCCTATGTGCTG TCAGTAAAAATGCAGGATCCGCAGTTTGCCGGGCAGACGAAAGAGCGTCTCTCTTCGCGT CAATGCGCGGCATTCGTTTCTGGCGTGGTGAAAGATGCCTTTATCCTGTGGCTGAACCAG AACGTTCAGGCGGCTGAACTGCTGGCGGAGATGGCGATTTCCAGCGCCCAGCGCCGTATG CGTGCGGCCAAAAAAGTGGTGCGTAAAAAGCTGACCAGCGGCCCGGCGTTGCCTGGCAAA CTGGCTGATTGTACCGCGCAGGACCTTAACCGTACCGAGCTGTTCCTTGTGGAAGGTGAC TCCGCAGGCGGATCTGCCAAGCAGGCGCGCGATCGCGAATATCAGGCGATCATGCCACTG AAAGGTAAGATCCTTAACACCTGGGAAGTCTCTTCCGACGAAGTGCTGGCTTCGCAGGAA GTGCACGATATTTCGGTAGCGATCGGTATCGATCCTGACAGCGACGATCTGAGCCAGCTT CGTTATGGCAAAATCTGTATCCTCGCGGATGCGGACTCTGATGGTCTGCACATTGCCACG CTGCTCTGCGCTTTGTTCGTAAAACATTTCCGCGCGTTGGTGAAACACGGTCACGTTTAC GTCGCACTGCCACCGCTCTACCGTATTGATCTCGGGAAAGAGGTTTATTACGCGCTGACG GAAGAAGAGAAAGAGGGCGTACTTGAGCAATTAAAACGCAAGAAAGGCAAGCCGAACGTC CAGCGTTTTAAAGGTCTGGGGGAAATGAACCCGATGCAATTGCGCGAAACCACGCTTGAT CCGAACACTCGCCGTCTGGTGCAGTTGACTATCGATGATGAAGACGATCAGCGTACTGAC GCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAA GAGAAAGGCGACATGGCGGAGATTGAGGTTTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P20083 UniProtKB Entry Name PARE_ECOLI GenBank Gene ID U28377 - General References
- Kato J, Nishimura Y, Imamura R, Niki H, Hiraga S, Suzuki H: New topoisomerase essential for chromosome segregation in E. coli. Cell. 1990 Oct 19;63(2):393-404. [Article]
- Springer AL, Schmid MB: Molecular characterization of the Salmonella typhimurium parE gene. Nucleic Acids Res. 1993 Apr 25;21(8):1805-9. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Peng H, Marians KJ: Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J Biol Chem. 1993 Nov 15;268(32):24481-90. [Article]
- Pitts SL, Liou GF, Mitchenall LA, Burgin AB, Maxwell A, Neuman KC, Osheroff N: Use of divalent metal ions in the DNA cleavage reaction of topoisomerase IV. Nucleic Acids Res. 2011 Jun;39(11):4808-17. doi: 10.1093/nar/gkr018. Epub 2011 Feb 7. [Article]
- Bellon S, Parsons JD, Wei Y, Hayakawa K, Swenson LL, Charifson PS, Lippke JA, Aldape R, Gross CH: Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase. Antimicrob Agents Chemother. 2004 May;48(5):1856-64. [Article]
- Breines DM, Ouabdesselam S, Ng EY, Tankovic J, Shah S, Soussy CJ, Hooper DC: Quinolone resistance locus nfxD of Escherichia coli is a mutant allele of the parE gene encoding a subunit of topoisomerase IV. Antimicrob Agents Chemother. 1997 Jan;41(1):175-9. [Article]
- Li Y, Stewart NK, Berger AJ, Vos S, Schoeffler AJ, Berger JM, Chait BT, Oakley MG: Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18832-7. doi: 10.1073/pnas.1008678107. Epub 2010 Oct 4. [Article]