Glucose-1-phosphate thymidylyltransferase
Details
- Name
- Glucose-1-phosphate thymidylyltransferase
- Synonyms
- 2.7.7.24
- dTDP-glucose pyrophosphorylase
- dTDP-glucose synthase
- Ep
- rfbA
- Gene Name
- rmlA
- Organism
- Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
- Amino acid sequence
>lcl|BSEQ0016605|Glucose-1-phosphate thymidylyltransferase MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLMLAGIRDILIISTPQD TPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGHDDCALVLGDNIFYGHDLP KLMEAAVNKESGATVFAYHVNDPERYGVVEFDQKGTAVSLEEKPLQPKSNYAVTGLYFYD NSVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMMGRGYAWLDTGTHQSLIEASNFI ATIEERQGLKVSCPEEIAFRKNFINAQQVIELAGPLSKNDYGKYLLKMVKGL
- Number of residues
- 292
- Molecular Weight
- 32453.085
- Theoretical pI
- 5.75
- GO Classification
- Functionsglucose-1-phosphate thymidylyltransferase activity / magnesium ion bindingProcessesdTDP-rhamnose biosynthetic process / extracellular polysaccharide biosynthetic process / O antigen biosynthetic process
- General Function
- Magnesium ion binding
- Specific Function
- Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Is also able to convert non natural substrates such as a wide array of alpha-D-hexopyranosyl, deoxy-alpha-D-glucopyranosyl, aminodeoxy-alpha-D-hexopyranosyl and acetamidodeoxy-alpha-D-hexopyranosyl phosphates to their corresponding dTDP- and UDP-nucleotide sugars.
- Pfam Domain Function
- NTP_transferase (PF00483)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016606|Glucose-1-phosphate thymidylyltransferase (rmlA) ATGAAAACGCGTAAGGGCATTATTTTAGCGGGGGGCTCCGGCACCCGTCTTTATCCGGTG ACCATGGCGGTAAGTAAGCAATTGCTACCAATTTATGATAAACCGATGATTTACTATCCC CTTTCCACGCTTATGCTGGCAGGCATTCGGGATATCCTGATCATCAGTACGCCACAGGAC ACGCCGCGTTTTCAACAACTGCTGGGAGACGGCAGCCAGTGGGGGCTGAATCTTCAATAT AAAGTACAGCCAAGCCCGGATGGCTTAGCACAGGCGTTTATTATTGGTGAAGAGTTCATT GGTCATGATGATTGTGCATTAGTGCTGGGTGACAATATCTTCTATGGTCATGATTTACCA AAGTTAATGGAAGCTGCCGTTAATAAAGAAAGTGGTGCTACCGTCTTCGCTTATCATGTA AACGATCCGGAGCGCTACGGTGTGGTTGAGTTTGACCAAAAGGGCACAGCCGTTAGTCTG GAAGAAAAACCATTACAACCGAAGAGTAATTACGCGGTAACGGGGCTGTATTTTTATGAT AATAGCGTGGTGGAGATGGCGAAAAATCTTAAGCCTTCCGCTCGCGGTGAGTTAGAAATC ACGGATATTAACCGTATCTATATGGAGCAGGGAAGATTGTCTGTCGCTATGATGGGGCGC GGTTATGCCTGGCTGGATACAGGGACGCATCAGAGTTTGATAGAGGCCAGTAATTTTATT GCAACCATCGAAGAACGCCAGGGGCTAAAAGTGTCCTGCCCGGAAGAGATCGCATTTCGT AAAAATTTTATAAATGCACAACAGGTTATAGAACTGGCCGGGCCATTATCAAAAAATGAT TATGGCAAATATTTGCTGAAGATGGTGAAAGGTTTATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P26393 UniProtKB Entry Name RMLA_SALTY GenBank Protein ID 47891 GenBank Gene ID X56793 - General References
- Jiang XM, Neal B, Santiago F, Lee SJ, Romana LK, Reeves PR: Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2). Mol Microbiol. 1991 Mar;5(3):695-713. [Article]
- McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001 Oct 25;413(6858):852-6. [Article]
- Lindquist L, Kaiser R, Reeves PR, Lindberg AA: Purification, characterization and HPLC assay of Salmonella glucose-1-phosphate thymidylyl-transferase from the cloned rfbA gene. Eur J Biochem. 1993 Feb 1;211(3):763-70. [Article]
- Barton WA, Lesniak J, Biggins JB, Jeffrey PD, Jiang J, Rajashankar KR, Thorson JS, Nikolov DB: Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization. Nat Struct Biol. 2001 Jun;8(6):545-51. [Article]