Cyclomaltodextrin glucanotransferase
Details
- Name
- Cyclomaltodextrin glucanotransferase
- Synonyms
- 2.4.1.19
- CGTase
- Cyclodextrin-glycosyltransferase
- Gene Name
- amyA
- Organism
- Thermoanaerobacterium thermosulfurigenes
- Amino acid sequence
>lcl|BSEQ0016550|Cyclomaltodextrin glucanotransferase MKKTFKLILVLMLSLTLVFGLTAPIQAASDTAVSNVVNYSTDVIYQIVTDRFVDGNTSNN PTGDLYDPTHTSLKKYFGGDWQGIINKINDGYLTGMGVTAIWISQPVENIYAVLPDSTFG GSTSYHGYWARDFKRTNPYFGSFTDFQNLINTAHAHNIKVIIDFAPNHTSPASETDPTYA ENGRLYDNGTLLGGYTNDTNGYFHHYGGTDFSSYEDGIYRNLFDLADLNQQNSTIDSYLK SAIKVWLDMGIDGIRLDAVKHMPFGWQKNFMDSILSYRPVFTFGEWFLGTNEIDVNNTYF ANESGMSLLDFRFSQKVRQVFRDNTDTMYGLDSMIQSTASDYNFINDMVTFIDNHDMDRF YNGGSTRPVEQALAFTLTSRGVPAIYYGTEQYMTGNGDPYNRAMMTSFNTSTTAYNVIKK LAPLRKSNPAIAYGTTQQRWINNDVYIYERKFGNNVALVAINRNLSTSYNITGLYTALPA GTYTDVLGGLLNGNSISVASDGSVTPFTLSAGEVAVWQYVSSSNSPLIGHVGPTMTKAGQ TITIDGRGFGTTSGQVLFGSTAGTIVSWDDTEVKVKVPSVTPGKYNISLKTSSGATSNTY NNINILTGNQICVRFVVNNASTVYGENVYLTGNVAELGNWDTSKAIGPMFNQVVYQYPTW YYDVSVPAGTTIQFKFIKKNGNTITWEGGSNHTYTVPSSSTGTVIVNWQQ
- Number of residues
- 710
- Molecular Weight
- 78416.955
- Theoretical pI
- 5.44
- GO Classification
- Functionscyclomaltodextrin glucanotransferase activity / metal ion binding / starch bindingProcessescarbohydrate metabolic processComponentsextracellular region
- General Function
- Starch binding
- Specific Function
- Degrades starch to alpha-, beta-, and gamma-cyclodextrins, as well as linear sugars.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Secreted
- Gene sequence
>lcl|BSEQ0003493|2133 bp ATGAAAAAAACGTTTAAATTGATATTGGTGCTGATGCTTTCACTTACACTTGTTTTTGGA TTGACAGCACCAATACAGGCAGCTTCTGATACAGCAGTAAGTAATGTTGTCAATTACTCG ACGGATGTAATTTATCAGATTGTCACTGATAGATTTGTTGATGGAAATACCAGCAACAAT CCAACTGGTGATTTGTATGATCCAACACATACAAGTCTGAAAAAGTACTTTGGTGGAGAC TGGCAGGGTATTATTAACAAGATAAATGATGGTTATCTAACAGGAATGGGTGTAACAGCA ATTTGGATATCTCAGCCTGTAGAAAATATTTATGCAGTCTTACCTGATTCAACTTTTGGT GGAAGTACATCGTACCATGGTTATTGGGCTCGTGATTTTAAGAGAACAAATCCATACTTT GGAAGCTTTACTGATTTTCAAAATTTGATTAATACAGCTCATGCACATAACATAAAAGTA ATTATCGATTTTGCACCAAATCATACTTCACCTGCATCTGAAACCGATCCGACTTATGCT GAAAATGGAAGGCTATACGACAATGGAACATTACTTGGTGGGTATACAAATGATACAAAC GGGTATTTTCATCATTATGGAGGAACAGATTTTTCATCTTATGAAGATGGCATTTATCGT AACTTATTTGATTTAGCAGATTTAAATCAACAGAATAGTACAATCGATTCATACTTAAAA TCAGCAATTAAGGTGTGGCTCGATATGGGAATAGACGGTATACGTCTAGATGCTGTAAAA CATATGCCGTTTGGATGGCAGAAGAATTTTATGGATAGCATATTGAGCTATAGGCCTGTA TTTACATTTGGAGAGTGGTTTCTTGGAACGAATGAAATTGATGTAAATAACACATACTTT GCAAATGAAAGCGGTATGAGTCTTTTGGACTTTAGGTTTTCTCAAAAAGTGAGGCAGGTA TTTAGGGATAATACGGATACTATGTATGGACTTGATTCAATGATTCAATCAACTGCATCT GATTATAATTTTATAAATGATATGGTTACTTTTATTGATAATCATGATATGGATAGATTC TATAATGGTGGTTCTACTCGTCCAGTTGAACAAGCATTAGCATTTACTTTGACTTCGAGG GGAGTACCTGCTATATATTATGGAACAGAACAGTATATGACAGGCAATGGAGACCCTTAT AATAGAGCTATGATGACCTCATTTAATACATCAACTACAGCATATAATGTAATTAAAAAA CTTGCTCCTTTGCGCAAATCTAATCCTGCAATTGCTTATGGTACTACTCAGCAACGTTGG ATAAACAATGATGTTTATATATATGAAAGAAAATTTGGGAATAATGTTGCTCTTGTTGCT ATAAATAGAAATCTTTCAACAAGTTATAATATAACAGGACTGTATACAGCGCTTCCTGCT GGTACTTATACTGATGTTCTTGGTGGACTTTTAAATGGTAATAGTATTTCTGTCGCGAGT GATGGTTCTGTAACACCATTTACACTTAGTGCCGGTGAAGTTGCAGTATGGCAGTATGTA AGTTCAAGTAATTCTCCGTTGATAGGACATGTTGGGCCAACAATGACAAAAGCAGGGCAA ACTATAACAATAGATGGAAGAGGTTTTGGTACGACATCAGGACAAGTTTTGTTTGGTTCG ACGGCTGGTACTATTGTCTCTTGGGATGATACAGAGGTAAAAGTAAAAGTTCCTTCGGTG ACACCTGGTAAATATAATATATCATTAAAAACTTCAAGTGGTGCTACAAGCAATACTTAT AATAATATTAACATTCTTACAGGCAATCAGATATGTGTTAGATTTGTTGTAAATAATGCA AGTACAGTGTATGGAGAGAATGTTTATCTTACTGGTAATGTAGCTGAATTGGGAAACTGG GATACGTCAAAAGCAATAGGACCAATGTTTAATCAGGTAGTATATCAGTATCCTACATGG TATTATGATGTAAGTGTTCCAGCGGGTACAACAATACAATTTAAGTTTATAAAGAAAAAT GGCAATACGATAACATGGGAAGGTGGTAGTAACCATACTTATACAGTTCCATCATCTAGT ACAGGTACTGTAATTGTAAACTGGCAACAATAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P26827 UniProtKB Entry Name CDGT_THETU GenBank Protein ID 1004352 GenBank Gene ID M57692 - General References
- Bahl H, Burchhardt G, Spreinat A, Haeckel K, Wienecke A, Schmidt B, Antranikian G: alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison of other alpha-amylases. Appl Environ Microbiol. 1991 May;57(5):1554-9. [Article]
- Bahl H, Burchhardt G, Wienecke A: Nucleotide sequence of two Clostridium thermosulfurogenes EM1 genes homologous to Escherichia coli genes encoding integral membrane components of binding protein-dependent transport systems. FEMS Microbiol Lett. 1991 Jun 1;65(1):83-7. [Article]
- Wind RD, Liebl W, Buitelaar RM, Penninga D, Spreinat A, Dijkhuizen L, Bahl H: Cyclodextrin formation by the thermostable alpha-amylase of Thermoanaerobacterium thermosulfurigenes EM1 and reclassification of the enzyme as a cyclodextrin glycosyltransferase. Appl Environ Microbiol. 1995 Apr;61(4):1257-65. [Article]
- Knegtel RM, Wind RD, Rozeboom HJ, Kalk KH, Buitelaar RM, Dijkhuizen L, Dijkstra BW: Crystal structure at 2.3 A resolution and revised nucleotide sequence of the thermostable cyclodextrin glycosyltransferase from Thermonanaerobacterium thermosulfurigenes EM1. J Mol Biol. 1996 Mar 1;256(3):611-22. [Article]
- Wind RD, Uitdehaag JC, Buitelaar RM, Dijkstra BW, Dijkhuizen L: Engineering of cyclodextrin product specificity and pH optima of the thermostable cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1. J Biol Chem. 1998 Mar 6;273(10):5771-9. [Article]