GDP-mannose mannosyl hydrolase

Details

Name
GDP-mannose mannosyl hydrolase
Synonyms
  • 3.6.1.-
  • Colanic acid biosynthesis protein WcaH
  • GDPMH
  • nudD
  • wcaH
  • yefC
Gene Name
gmm
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0003482|GDP-mannose mannosyl hydrolase
MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGYWFVPGGRVQKDETLEA
AFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHYVVLGFRFRVSEEELLLPD
EQHDDYRWLTSDALLASDNVHANSRAYFLAEKRTGVPGL
Number of residues
159
Molecular Weight
18273.34
Theoretical pI
4.83
GO Classification
Functions
GDP-glucosidase activity / GDP-mannose mannosyl hydrolase activity / magnesium ion binding / manganese ion binding
Processes
lipopolysaccharide biosynthetic process
General Function
Manganese ion binding
Specific Function
Hydrolyzes both GDP-mannose and GDP-glucose. Could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. Might also be involved in the biosynthesis of the slime polysaccharide colanic acid.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016547|GDP-mannose mannosyl hydrolase (gmm)
ATGTTTTTACGTCAGGAAGACTTTGCCACGGTAGTGCGCTCCACTCCGCTTGTCTCTCTC
GACTTTATTGTCGAGAACAGTCGCGGCGAGTTTCTGCTTGGCAAAAGAACCAACCGCCCG
GCGCAGGGTTACTGGTTTGTGCCGGGAGGGCGCGTGCAGAAAGACGAAACGCTGGAAGCC
GCATTTGAGCGGCTGACGATGGCGGAACTGGGGCTGCGTTTGCCGATAACAGCAGGCCAG
TTTTACGGTGTCTGGCAGCACTTTTATGACGATAACTTCTCTGGCACGGATTTCACCACT
CACTATGTGGTGCTCGGTTTTCGCTTCAGAGTATCGGAAGAAGAGCTGTTACTGCCGGAT
GAGCAGCATGACGATTACCGCTGGCTGACGTCGGACGCGCTGCTCGCCAGTGATAATGTT
CATGCTAACAGCCGCGCCTATTTTCTCGCTGAGAAGCGTACCGGAGTACCCGGATTATGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP32056
UniProtKB Entry NameGMM_ECOLI
GenBank Gene IDU38473
General References
  1. Aoyama K, Haase AM, Reeves PR: Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12. Mol Biol Evol. 1994 Nov;11(6):829-38. [Article]
  2. Stevenson G, Andrianopoulos K, Hobbs M, Reeves PR: Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J Bacteriol. 1996 Aug;178(16):4885-93. [Article]
  3. Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.: A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):379-92. [Article]
  4. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  5. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  6. Frick DN, Townsend BD, Bessman MJ: A novel GDP-mannose mannosyl hydrolase shares homology with the MutT family of enzymes. J Biol Chem. 1995 Oct 13;270(41):24086-91. [Article]
  7. Legler PM, Massiah MA, Bessman MJ, Mildvan AS: GDP-mannose mannosyl hydrolase catalyzes nucleophilic substitution at carbon, unlike all other Nudix hydrolases. Biochemistry. 2000 Jul 25;39(29):8603-8. [Article]
  8. Legler PM, Massiah MA, Mildvan AS: Mutational, kinetic, and NMR studies of the mechanism of E. coli GDP-mannose mannosyl hydrolase, an unusual Nudix enzyme. Biochemistry. 2002 Sep 3;41(35):10834-48. [Article]
  9. Xia Z, Azurmendi HF, Lairson LL, Withers SG, Gabelli SB, Bianchet MA, Amzel LM, Mildvan AS: Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction. Biochemistry. 2005 Jun 28;44(25):8989-97. [Article]
  10. Gabelli SB, Bianchet MA, Azurmendi HF, Xia Z, Sarawat V, Mildvan AS, Amzel LM: Structure and mechanism of GDP-mannose glycosyl hydrolase, a Nudix enzyme that cleaves at carbon instead of phosphorus. Structure. 2004 Jun;12(6):927-35. [Article]
  11. Gabelli SB, Azurmendi HF, Bianchet MA, Amzel LM, Mildvan AS: X-ray, NMR, and mutational studies of the catalytic cycle of the GDP-mannose mannosyl hydrolase reaction. Biochemistry. 2006 Sep 26;45(38):11290-303. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB04315Guanosine-5'-DiphosphateexperimentalunknownDetails