50S ribosomal protein L4
Details
- Name
- 50S ribosomal protein L4
- Synonyms
- rpl4
- Gene Name
- rplD
- Organism
- Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
- Amino acid sequence
>lcl|BSEQ0012593|50S ribosomal protein L4 MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGTRAQKTRAEVSGSGKKPWR QKGTGRARAGDIKSPIWRSGGTTFAAKPQDHSQKVNKKMYRGAIKSILSELVRQDRLVVV EKFELDAPKTKVLVQKLKDLAVEDALIITASLDENLFLAARNLYKVDVRDVQGIDPVSLI AFDKVIVTVDAVKQIEEILA
- Number of residues
- 200
- Molecular Weight
- 21954.185
- Theoretical pI
- 10.28
- GO Classification
- FunctionsrRNA binding / structural constituent of ribosomeProcessesDNA-templated transcription, termination / regulation of transcription, DNA-templated / regulation of translation / response to antibiotic / translationComponentsribosome
- General Function
- Structural constituent of ribosome
- Specific Function
- One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity).Protein L4 is a both a transcriptional repressor and a translational repressor protein. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader. L4 controls the translation of the S10 operon by binding to its mRNA (By similarity).This protein when expressed in E.coli represses both transcription and translation of the endogenous S10 operon. As the H.influenzae S10 leader can be regulated in vitro by the E.coli L4 protein this strongly suggests the endogenous protein controls its own S10 operon in a similar fashion.Forms part of the polypeptide exit tunnel.
- Pfam Domain Function
- Ribosomal_L4 (PF00573)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0012594|50S ribosomal protein L4 (rplD) ATGGAATTACAAGTTGTAGGTGCAAACGCACTCACTGTTTCTGAAACTACCTTCGGACGT GAGTTTAACGAAGCGTTGATCCACCAAGTTGTTGTTGCTTATGCAGCAGGTGCGCGTCAA GGTACTCGTGCACAAAAAACTCGTGCTGAAGTGTCTGGTTCAGGTAAAAAACCTTGGCGT CAAAAAGGTACAGGTCGTGCTCGTGCTGGTGATATCAAATCACCAATCTGGCGTTCTGGT GGTACAACCTTCGCGGCTAAACCACAAGATCACAGCCAAAAAGTGAACAAGAAAATGTAC CGTGGTGCTATCAAAAGCATTCTTTCTGAATTAGTTCGTCAAGACCGTTTGGTTGTGGTG GAAAAATTTGAATTAGATGCACCAAAGACTAAAGTTTTAGTACAAAAATTAAAAGATTTA GCCGTTGAAGATGCGTTAATTATCACAGCAAGTTTAGATGAAAATCTATTCTTAGCAGCA CGTAACTTATATAAAGTTGATGTCCGTGATGTTCAAGGTATCGATCCAGTTAGCTTGATC GCTTTCGATAAGGTGATTGTTACTGTTGATGCTGTGAAACAAATTGAGGAGATCCTAGCA TGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P44345 UniProtKB Entry Name RL4_HAEIN GenBank Protein ID 3212176 GenBank Gene ID L42023 - General References
- Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM, et al.: Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496-512. [Article]
- Zengel JM, Vorozheikina D, Li X, Lindahl L: Regulation of the Escherichia coli S10 ribosomal protein operon by heterologous L4 ribosomal proteins. Biochem Cell Biol. 1995 Nov-Dec;73(11-12):1105-12. [Article]
- Allen T, Shen P, Samsel L, Liu R, Lindahl L, Zengel JM: Phylogenetic analysis of L4-mediated autogenous control of the S10 ribosomal protein operon. J Bacteriol. 1999 Oct;181(19):6124-32. [Article]
- Peric M, Bozdogan B, Jacobs MR, Appelbaum PC: Effects of an efflux mechanism and ribosomal mutations on macrolide susceptibility of Haemophilus influenzae clinical isolates. Antimicrob Agents Chemother. 2003 Mar;47(3):1017-22. [Article]