50S ribosomal protein L4

Details

Name
50S ribosomal protein L4
Synonyms
  • rpl4
Gene Name
rplD
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Amino acid sequence
>lcl|BSEQ0012593|50S ribosomal protein L4
MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGTRAQKTRAEVSGSGKKPWR
QKGTGRARAGDIKSPIWRSGGTTFAAKPQDHSQKVNKKMYRGAIKSILSELVRQDRLVVV
EKFELDAPKTKVLVQKLKDLAVEDALIITASLDENLFLAARNLYKVDVRDVQGIDPVSLI
AFDKVIVTVDAVKQIEEILA
Number of residues
200
Molecular Weight
21954.185
Theoretical pI
10.28
GO Classification
Functions
rRNA binding / structural constituent of ribosome
Processes
DNA-templated transcription, termination / regulation of transcription, DNA-templated / regulation of translation / response to antibiotic / translation
Components
ribosome
General Function
Structural constituent of ribosome
Specific Function
One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity).Protein L4 is a both a transcriptional repressor and a translational repressor protein. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader. L4 controls the translation of the S10 operon by binding to its mRNA (By similarity).This protein when expressed in E.coli represses both transcription and translation of the endogenous S10 operon. As the H.influenzae S10 leader can be regulated in vitro by the E.coli L4 protein this strongly suggests the endogenous protein controls its own S10 operon in a similar fashion.Forms part of the polypeptide exit tunnel.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0012594|50S ribosomal protein L4 (rplD)
ATGGAATTACAAGTTGTAGGTGCAAACGCACTCACTGTTTCTGAAACTACCTTCGGACGT
GAGTTTAACGAAGCGTTGATCCACCAAGTTGTTGTTGCTTATGCAGCAGGTGCGCGTCAA
GGTACTCGTGCACAAAAAACTCGTGCTGAAGTGTCTGGTTCAGGTAAAAAACCTTGGCGT
CAAAAAGGTACAGGTCGTGCTCGTGCTGGTGATATCAAATCACCAATCTGGCGTTCTGGT
GGTACAACCTTCGCGGCTAAACCACAAGATCACAGCCAAAAAGTGAACAAGAAAATGTAC
CGTGGTGCTATCAAAAGCATTCTTTCTGAATTAGTTCGTCAAGACCGTTTGGTTGTGGTG
GAAAAATTTGAATTAGATGCACCAAAGACTAAAGTTTTAGTACAAAAATTAAAAGATTTA
GCCGTTGAAGATGCGTTAATTATCACAGCAAGTTTAGATGAAAATCTATTCTTAGCAGCA
CGTAACTTATATAAAGTTGATGTCCGTGATGTTCAAGGTATCGATCCAGTTAGCTTGATC
GCTTTCGATAAGGTGATTGTTACTGTTGATGCTGTGAAACAAATTGAGGAGATCCTAGCA
TGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP44345
UniProtKB Entry NameRL4_HAEIN
GenBank Protein ID3212176
GenBank Gene IDL42023
General References
  1. Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM, et al.: Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496-512. [Article]
  2. Zengel JM, Vorozheikina D, Li X, Lindahl L: Regulation of the Escherichia coli S10 ribosomal protein operon by heterologous L4 ribosomal proteins. Biochem Cell Biol. 1995 Nov-Dec;73(11-12):1105-12. [Article]
  3. Allen T, Shen P, Samsel L, Liu R, Lindahl L, Zengel JM: Phylogenetic analysis of L4-mediated autogenous control of the S10 ribosomal protein operon. J Bacteriol. 1999 Oct;181(19):6124-32. [Article]
  4. Peric M, Bozdogan B, Jacobs MR, Appelbaum PC: Effects of an efflux mechanism and ribosomal mutations on macrolide susceptibility of Haemophilus influenzae clinical isolates. Antimicrob Agents Chemother. 2003 Mar;47(3):1017-22. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB01321JosamycininvestigationalyesinhibitorDetails