Purine nucleoside phosphorylase 2

Details

Name
Purine nucleoside phosphorylase 2
Synonyms
  • 2.4.2.1
  • Inosine-guanosine phosphorylase
  • pndA
  • PNP II
  • Purine nucleoside phosphorylase II
  • Xanthosine phosphorylase
Gene Name
xapA
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0003089|Purine nucleoside phosphorylase 2
MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIENAVAISYEKLPGFPVS
TVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIRTFKLLGCELLFCTNAAGS
LRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSLANAYDAEYRALLQKVAKEEG
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCDLKVVAVSAITNM
AEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKIA
Number of residues
277
Molecular Weight
29834.38
Theoretical pI
6.5
GO Classification
Functions
guanosine phosphorylase activity / identical protein binding / inosine nucleosidase activity / purine-nucleoside phosphorylase activity
Processes
deoxyguanosine catabolic process / deoxyinosine catabolic process / guanosine catabolic process / inosine catabolic process / nucleobase-containing small molecule interconversion / nucleobase-containing small molecule metabolic process / protein hexamerization / purine nucleoside catabolic process / purine-containing compound salvage / xanthosine catabolic process
Components
cytoplasm
General Function
Purine-nucleoside phosphorylase activity
Specific Function
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. This protein can degrade all purine nucleosides including xanthosine, inosine and guanosine, but cannot cleave adenosine, deoxyadenosine or hypoxanthine arabinoside. Has a preference for the neutral over the monoanionic form of xanthosine.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016471|Purine nucleoside phosphorylase 2 (xapA)
ATGTCTCAGGTTCAATTTTCTCATAACCCGCTGTTTTGCATAGATATTATCAAGACTTAT
AAACCTGATTTCACGCCACGAGTGGCCTTTATTTTAGGTTCCGGGCTCGGCGCGCTGGCC
GATCAGATTGAGAACGCTGTCGCAATTTCCTACGAAAAGCTGCCGGGCTTCCCGGTAAGT
ACGGTACATGGTCATGCGGGGGAGTTAGTGCTGGGCCATCTCCAGGGCGTACCGGTGGTA
TGTATGAAAGGTCGCGGACATTTCTACGAAGGTCGTGGAATGACCATTATGACTGACGCA
ATTCGTACCTTTAAGCTGCTGGGCTGCGAGCTACTGTTCTGCACCAATGCGGCAGGCTCG
CTGCGTCCGGAAGTGGGGGCAGGCAGCCTGGTCGCATTGAAAGATCATATCAACACCATG
CCCGGTACGCCGATGGTGGGTCTTAACGATGATCGTTTTGGAGAGCGCTTCTTCTCACTG
GCAAATGCCTACGATGCGGAATACCGCGCACTGTTACAAAAAGTGGCGAAAGAAGAGGGT
TTCCCTCTGACGGAGGGCGTGTTCGTCTCGTATCCGGGGCCGAATTTCGAGACTGCGGCG
GAAATTCGCATGATGCAAATTATTGGTGGGGATGTTGTTGGTATGTCTGTGGTGCCTGAG
GTTATTTCAGCTCGCCATTGCGACCTTAAAGTCGTTGCGGTCTCTGCGATTACCAATATG
GCGGAAGGTTTAAGCGATGTGAAATTGTCACATGCGCAAACGCTGGCGGCTGCGGAGCTC
TCTAAGCAGAACTTCATCAACCTTATTTGTGGCTTTCTGCGCAAAATTGCCTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP45563
UniProtKB Entry NameXAPA_ECOLI
GenBank Protein ID929584
GenBank Gene IDX73828
General References
  1. Seeger C, Poulsen C, Dandanell G: Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli. J Bacteriol. 1995 Oct;177(19):5506-16. [Article]
  2. Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Buxton RS, Hammer-Jespersen K, Valentin-Hansen P: A second purine nucleoside phosphorylase in Escherichia coli K-12. I. Xanthosine phosphorylase regulatory mutants isolated as secondary-site revertants of a deoD mutant. Mol Gen Genet. 1980;179(2):331-40. [Article]
  6. Hammer-Jespersen K, Buxton RS, Hansen TD: A second purine nucleoside phosphorylase in Escherichia coli K-12. II. Properties of xanthosine phosphorylase and its induction by xanthosine. Mol Gen Genet. 1980;179(2):341-8. [Article]
  7. Koszalka GW, Vanhooke J, Short SA, Hall WW: Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12. J Bacteriol. 1988 Aug;170(8):3493-8. [Article]
  8. Jorgensen C, Dandanell G: Isolation and characterization of mutations in the Escherichia coli regulatory protein XapR. J Bacteriol. 1999 Jul;181(14):4397-403. [Article]
  9. Shimaoka M, Takenaka Y, Mihara Y, Kurahashi O, Kawasaki H, Matsui H: Effects of xapA and guaA disruption on inosine accumulation in Escherichia coli. Biosci Biotechnol Biochem. 2006 Dec;70(12):3069-72. Epub 2006 Dec 7. [Article]
  10. Dandanell G, Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M: Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene. J Mol Biol. 2005 Apr 22;348(1):113-25. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB02134XanthineexperimentalunknownDetails
DB02377GuanineexperimentalunknownDetails