L-hydantoinase

Details

Name
L-hydantoinase
Synonyms
  • 3.5.2.-
  • Non-ATP-dependent L-selective hydantoinase
Gene Name
lhyD
Organism
Arthrobacter aurescens
Amino acid sequence
>lcl|BSEQ0011268|L-hydantoinase
MFDVIVKNCRLVSSDGITEADILVKDGKVAAISADTSDVEASRTIDAGGKFVMPGVVDEH
VHIIDMDLKNRYGRFELDSESAAVGGITTIIEMPITFPPTTTLDAFLEKKKQAGQRLKVD
FALYGGGVPGNLPEIRKMHDAGAVGFKSMMAASVPGMFDAVSDGELFEIFQEIAACGSVI
VVHAENETIIQALQKQIKAAGGKDMAAYEASQPVFQENEAIQRALLLQKEAGCRLIVLHV
SNPDGVELIHQAQSEGQDVHCESGPQYLNITTDDAERIGPYMKVAPPVRSAEMNIRLWEQ
LENGLIDTLGSDHGGHPVEDKEPGWKDVWKAGNGALGLETSLPMMLTNGVNKGRLSLERL
VEVMCEKPAKLFGIYPQKGTLQVGSDADLLILDLDIDTKVDASQFRSLHKYSPFDGMPVT
GAPVLTMVRGTVVAEKGEVLVEQGFGQFVTRRNYEASK
Number of residues
458
Molecular Weight
49596.275
Theoretical pI
4.66
GO Classification
Functions
allantoinase activity / cobalt ion binding / zinc ion binding
Processes
allantoin catabolic process
General Function
Zinc ion binding
Specific Function
Rather more predominant for the cleavage of aryl- than for alkyl-hydantoin derivatives. The stereoselectivity of this enzyme depends on the substrate used for bioconversion: strictly L-selective for the cleavage of D,L-5-indolylmethylhydantoin, but D-selective for the hydrolysis of D,L-methylthioethylhydantoin.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasmic
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP81006
UniProtKB Entry NameHYDL_ARTAU
General References
  1. May O, Habenicht A, Mattes R, Syldatk C, Siemann M: Molecular evolution of hydantoinases. Biol Chem. 1998 Jun;379(6):743-7. [Article]
  2. May O, Siemann M, Pietzsch M, Kiess M, Mattes R, Syldatk C: Substrate-dependent enantioselectivity of a novel hydantoinase from Arthrobacter aurescens DSM 3745: purification and characterization as new member of cyclic amidases. J Biotechnol. 1998 Mar 26;61(1):1-13. [Article]
  3. May O, Siemann M, Syldatk C, Niefind K, Schomburg D: Crystallization and preliminary X-ray analysis of a hydantoinase from Arthrobacter aurescens DSM 3745. Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1209-10. [Article]
  4. Abendroth J, Niefind K, May O, Siemann M, Syldatk C, Schomburg D: The structure of L-hydantoinase from Arthobacter aurescens leads to an understanding of dihydropyrimidinase substrate and enantio specificity. Biochemistry. 2002 Jul 9;41(27):8589-97. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03801Lysine Nz-Carboxylic AcidexperimentalunknownDetails