DNA topoisomerase 1

Details

Name

DNA topoisomerase 1

Synonyms

• 5.99.1.2
• DNA topoisomerase I
• Omega-protein
• Relaxing enzyme
• Swivelase
• Untwisting enzyme

Gene Name

topA

Organism

Staphylococcus aureus

Amino acid sequence

>lcl|BSEQ0013380|DNA topoisomerase 1
MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGK
GPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKE
SFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDREN
EIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITTALDGDQFEITN
VTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMR
TDSTRISDTAKAEAKQYITNKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSF
LTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETK
DDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAP
TIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDIT
WRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNF
PDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPK
CNQYLVENKKGKTTQVICSNCDYKEAAQK

Number of residues

689

Molecular Weight

79099.16

Theoretical pI

Not Available

GO Classification

Functions

DNA binding / DNA topoisomerase type I activity / magnesium ion binding

Processes

DNA topological change

Components

chromosome

General Function

Magnesium ion binding

Specific Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Pfam Domain Function

• Toprim (PF01751)
• Topoisom_bac (PF01131)
• zf-C4_Topoisom (PF01396)

Transmembrane Regions

Not Available

Cellular Location

Not Available

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	Q06AK7
UniProtKB Entry Name	TOP1_STAAU

General References

Not Available

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB00487	Pefloxacin	approved	unknown	inhibitor	Details
DB01051	Novobiocin	approved, investigational, vet_approved, withdrawn	unknown	inhibitor	Details