Ribonucleoside-diphosphate reductase 2 subunit alpha
Details
- Name
- Ribonucleoside-diphosphate reductase 2 subunit alpha
- Synonyms
- 1.17.4.1
- R1E protein
- Ribonucleotide reductase 2
- Gene Name
- nrdE
- Organism
- Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
- Amino acid sequence
>lcl|BSEQ0016556|Ribonucleoside-diphosphate reductase 2 subunit alpha MATTTPERVMQETMDYHALNAMLNLYDKAGHIQFDKDQQAIDAFFATHVRPHSVTFASQH ERLGTLVREGYYDDAVLARYDRAFVLRLFEHAHASGFRFQTFLGAWKFYTSYTLKTFDGK RYLEHFEDRVTMVALTLAQGDETLATQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFRLA KENAQMALFSPYDIQRRYGKPFGDIAISERYDELIADPHVRKTYINARDFFQTLAEIQFE SGYPYIMFEDTVNRANPIAGRINMSNLCSEILQVNSASRYDDNLDYTHIGHDISCNLGSL NIAHVMDSPDIGRTVETAIRGLTAVSDMSHIRSVPSIAAGNAASHAIGLGQMNLHGYLAR EGIAYGSPEALDFTNLYFYTITWHAVHTSMRLARERGKTFAGFAQSRYASGDYFTQYLQD DWQPKTAKVRALFARSGITLPTREMWLKLRDDVMRYGIYNQNLQAVPPTGSISYINHATS SIHPIVAKIEIRKEGKTGRVYYPAPFMTNENLDMYQDAYDIGPEKIIDTYAEATRHVDQG LSLTLFFPDTATTRDINKAQIYAWRKGIKSLYYIRLRQLALEGTEIEGCVSCAL
- Number of residues
- 714
- Molecular Weight
- 80586.595
- Theoretical pI
- 7.04
- GO Classification
- FunctionsATP binding / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptorProcessesDNA replication
- General Function
- Ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
- Specific Function
- Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016557|Ribonucleoside-diphosphate reductase 2 subunit alpha (nrdE) TTGGCAACAACTACCCCGGAGCGCGTAATGCAGGAAACCATGGATTACCACGCCCTGAAC GCGATGCTGAATCTTTACGATAAAGCAGGCCATATTCAGTTCGACAAGGACCAGCAGGCG ATCGACGCCTTCTTTGCCACCCACGTCCGCCCGCATTCCGTGACGTTTGCCAGCCAGCAT GAACGTCTGGGGACGCTGGTTCGGGAAGGGTATTACGATGACGCCGTCCTCGCGCGTTAC GACCGCGCCTTCGTCCTTCGCCTGTTCGAGCACGCCCATGCCAGCGGCTTTCGCTTCCAG ACGTTTCTTGGCGCCTGGAAGTTCTATACCAGTTACACGCTGAAAACCTTCGACGGCAAA CGTTATCTGGAACACTTTGAAGATCGGGTGACAATGGTGGCGTTGACGCTGGCGCAGGGT GACGAAACGCTGGCCACCCAACTGACCGATGAAATGCTTTCTGGTCGCTTTCAGCCCGCT ACCCCGACTTTTTTAAATTGCGGCAAACAGCAGCGTGGGGAACTGGTCTCCTGCTTCCTG CTCCGTATCGAAGACAACATGGAGTCGATCGGGCGGGCGGTGAATTCGGCGCTGCAACTC TCCAAACGCGGCGGCGGCGTCGCGTTTTTACTCTCCAATCTGCGCGAGGCGGGCGCGCCG ATCAAACGCATTGAGAATCAGTCTTCCGGCGTGATCCCGGTGATGAAAATGCTGGAAGAC GCGTTTTCGTATGCCAACCAACTTGGCGCGCGCCAGGGGGCCGGCGCGGTTTATCTCCAT GCGCACCATCCGGATATTCTGCGTTTTCTGGATACCAAACGAGAAAACGCTGACGAAAAA ATCCGGATCAAAACGCTCTCTCTCGGCGTGGTGATCCCGGATATCACCTTCCGGCTGGCG AAAGAAAACGCGCAAATGGCGCTCTTTTCGCCCTATGACATACAACGACGCTACGGCAAA CCGTTTGGCGATATCGCCATTAGCGAACGGTACGATGAATTAATTGCCGATCCGCACGTG CGCAAAACCTATATTAACGCCCGTGACTTTTTTCAAACACTGGCGGAGATTCAGTTCGAA TCCGGGTATCCCTACATCATGTTTGAAGATACGGTAAACCGCGCGAATCCCATTGCTGGT CGCATTAATATGAGCAACCTGTGCTCAGAAATTTTACAGGTCAATAGCGCTTCCCGTTAC GACGATAACCTTGACTATACCCACATCGGGCATGACATCTCCTGCAATCTCGGCTCGCTG AATATCGCTCACGTCATGGATTCACCGGACATTGGCCGTACCGTAGAAACCGCTATTCGC GGCCTGACGGCGGTGTCGGACATGAGCCATATACGCAGCGTGCCCTCAATAGCCGCCGGT AATGCCGCCTCTCATGCCATCGGTCTGGGCCAGATGAATCTGCATGGCTATCTGGCGAGG GAAGGTATTGCCTACGGTTCGCCGGAGGCGTTGGATTTCACCAATCTCTATTTTTACACC ATTACCTGGCATGCCGTGCATACTTCAATGCGGCTAGCCCGCGAACGCGGCAAAACCTTC GCCGGATTTGCGCAGTCGCGCTATGCCAGCGGCGACTATTTTACGCAGTATTTACAGGAC GACTGGCAACCGAAAACAGCGAAAGTCAGGGCGCTATTTGCCCGCAGCGGCATTACGCTG CCCACACGAGAAATGTGGCTAAAGCTGCGCGACGATGTGATGCGCTATGGCATCTATAAC CAAAATTTGCAGGCGGTGCCGCCGACCGGTTCGATTTCTTACATTAATCATGCGACCTCC AGCATTCATCCGATTGTGGCCAAAATTGAGATTCGCAAAGAGGGCAAAACCGGGCGTGTG TATTACCCCGCGCCGTTTATGACCAATGAAAACCTGGACATGTATCAGGATGCTTACGAT ATCGGTCCGGAAAAAATTATTGATACCTATGCCGAGGCCACGCGCCACGTCGATCAAGGG CTGTCGCTCACCCTGTTTTTCCCCGATACCGCCACGACCCGCGATATCAACAAGGCGCAG ATCTATGCCTGGCGAAAAGGTATTAAGTCCCTGTATTACATCCGGCTTCGCCAGTTGGCG CTGGAAGGTACTGAAATTGAAGGCTGCGTATCCTGCGCGCTATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q08698 UniProtKB Entry Name RIR3_SALTY GenBank Protein ID 1050838 GenBank Gene ID X73226 - General References
- Jordan A, Gibert I, Barbe J: Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase. J Bacteriol. 1994 Jun;176(11):3420-7. [Article]
- McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001 Oct 25;413(6858):852-6. [Article]
- Uppsten M, Farnegardh M, Jordan A, Eliasson R, Eklund H, Uhlin U: Structure of the large subunit of class Ib ribonucleotide reductase from Salmonella typhimurium and its complexes with allosteric effectors. J Mol Biol. 2003 Jun 27;330(1):87-97. [Article]
- Uppsten M, Farnegardh M, Domkin V, Uhlin U: The first holocomplex structure of ribonucleotide reductase gives new insight into its mechanism of action. J Mol Biol. 2006 Jun 2;359(2):365-77. Epub 2006 Mar 31. [Article]