Regucalcin

Details

Name

Regucalcin

Synonyms

• Gluconolactonase
• GNL
• RC
• Senescence marker protein 30
• SMP-30
• SMP30

Gene Name

RGN

Organism

Humans

Amino acid sequence

>lcl|BSEQ0009021|Regucalcin
MSSIKIECVLPENCRCGESPVWEEVSNSLLFVDIPAKKVCRWDSFTKQVQRVTMDAPVSS
VALRQSGGYVATIGTKFCALNWKEQSAVVLATVDNDKKNNRFNDGKVDPAGRYFAGTMAE
ETAPAVLERHQGALYSLFPDHHVKKYFDQVDISNGLDWSLDHKIFYYIDSLSYSVDAFDY
DLQTGQISNRRSVYKLEKEEQIPDGMCIDAEGKLWVACYNGGRVIRLDPVTGKRLQTVKL
PVDKTTSCCFGGKNYSEMYVTCARDGMDPEGLLRQPEAGGIFKITGLGVKGIAPYSYAG

Number of residues

299

Molecular Weight

33252.53

Theoretical pI

Not Available

GO Classification

Functions

calcium ion binding / enzyme regulator activity / gluconolactonase activity / zinc ion binding

Processes

aging / cellular calcium ion homeostasis / kidney development / L-ascorbic acid biosynthetic process / liver regeneration / negative regulation of apoptotic process / negative regulation of bone development / negative regulation of calcium-dependent ATPase activity / negative regulation of cyclic-nucleotide phosphodiesterase activity / negative regulation of DNA biosynthetic process / negative regulation of DNA catabolic process / negative regulation of epithelial cell proliferation / negative regulation of GTPase activity / negative regulation of leucine-tRNA ligase activity / negative regulation of nitric oxide biosynthetic process / negative regulation of phosphoprotein phosphatase activity / negative regulation of protein kinase activity / negative regulation of RNA biosynthetic process / negative regulation of sperm motility / positive regulation of ATPase activity / positive regulation of calcium-transporting ATPase activity / positive regulation of dUTP diphosphatase activity / positive regulation of fatty acid biosynthetic process / positive regulation of glucose metabolic process / positive regulation of GTPase activity / positive regulation of phosphatase activity / positive regulation of proteolysis involved in cellular protein catabolic process / positive regulation of superoxide dismutase activity / positive regulation of triglyceride biosynthetic process / regulation of calcium-mediated signaling / spermatogenesis

Components

cytoplasm / cytosol / nucleoplasm / nucleus

General Function

Zinc ion binding

Specific Function

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).

Pfam Domain Function

• SGL (PF08450)

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0013568|Regucalcin (RGN)
ATGGATGCCCCAGTCAGCTCCGTGGCTCTTCGCCAGTCGGGAGGCTATGTTGCCACCATT
GGAACAAAGTTCTGTGCTTTGAACTGGAAAGAACAATCAGCAGTTGTCTTGGCCACGGTG
GATAACGACAAGAAAAACAATCGCTTCAATGATGGGAAGGTGGATCCCGCCGGGAGGTAC
TTTGCTGGCACCATGGCTGAGGAAACAGCTCCAGCAGTTCTTGAGCGGCACCAGGGGGCC
CTGTACTCCCTCTTTCCTGATCACCACGTGAAAAAGTACTTTGACCAGGTGGACATTTCC
AATGGTTTGGATTGGTCGCTAGACCACAAAATCTTCTATTACATTGACAGCCTGTCCTAC
TCCGTGGATGCCTTTGACTATGACCTGCAGACAGGACAGATCTCCAACCGCAGAAGTGTT
TACAAGCTAGAAAAGGAAGAACAAATCCCAGATGGAATGTGTATTGATGCTGAGGGGAAG
CTCTGGGTGGCCTGTTACAATGGAGGAAGAGTGATTCGTTTAGATCCTGTGACAGGGAAA
AGACTTCAAACTGTGAAGTTGCCTGTTGATAAAACAACTTCATGCTGCTTTGGAGGGAAG
AATTACTCTGAAATGTATGTGACCTGCGCCCGGGATGGGATGGACCCCGAGGGTCTTTTG
AGGCAACCTGAAGCTGGTGGAATTTTCAAGATAACTGGTCTGGGGGTCAAAGGAATTGCT
CCCTACTCCTATGCGGGATGA

Chromosome Location

X

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	Q15493
UniProtKB Entry Name	RGN_HUMAN
HGNC ID	HGNC:9989

General References

1. Fujita T, Mandel JL, Shirasawa T, Hino O, Shirai T, Maruyama N: Isolation of cDNA clone encoding human homologue of senescence marker protein-30 (SMP30) and its location on the X chromosome. Biochim Biophys Acta. 1995 Sep 19;1263(3):249-52. [Article]
2. Misawa H, Yamaguchi M: Transcript heterogeneity of the human gene for Ca2+-binding protein regucalcin. Int J Mol Med. 2000 Mar;5(3):283-7. [Article]
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5. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
6. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
7. Chakraborti S, Bahnson BJ: Crystal structure of human senescence marker protein 30: insights linking structural, enzymatic, and physiological functions . Biochemistry. 2010 Apr 27;49(16):3436-44. doi: 10.1021/bi9022297. [Article]
8. Aizawa S, Senda M, Harada A, Maruyama N, Ishida T, Aigaki T, Ishigami A, Senda T: Structural basis of the gamma-lactone-ring formation in ascorbic acid biosynthesis by the senescence marker protein-30/gluconolactonase. PLoS One. 2013;8(1):e53706. doi: 10.1371/journal.pone.0053706. Epub 2013 Jan 22. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB11093	Calcium citrate	approved, investigational	no	ligand	Details
DB11348	Calcium Phosphate	approved	no	ligand	Details
DB14481	Calcium phosphate dihydrate	approved	no		Details