Chondroitinase-AC
Details
- Name
- Chondroitinase-AC
- Synonyms
- 4.2.2.5
- chnAC
- Chondroitin sulfate AC lyase
- Chondroitin-AC eliminase
- Chondroitin-AC lyase
- Gene Name
- cslA
- Organism
- Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / NCIB 9290)
- Amino acid sequence
>lcl|BSEQ0010992|Chondroitinase-AC MKKLFVTCIVFFSILSPALLIAQQTGTAELIMKRVMLDLKKPLRNMDKVAEKNLNTLQPD GSWKDVPYKDDAMTNWLPNNHLLQLETIIQAYIEKDSHYYGDDKVFDQISKAFKYWYDSD PKSRNWWHNEIATPQALGEMLILMRYGKKPLDEALVHKLTERMKRGEPEKKTGANKTDIA LHYFYRALLTSDEALLSFAVKELFYPVQFVHYEEGLQYDYSYLQHGPQLQISSYGAVFIT GVLKLANYVRDTPYALSTEKLAIFSKYYRDSYLKAIRGSYMDFNVEGRGVSRPDILNKKA EKKRLLVAKMIDLKHTEEWADAIARTDSTVAAGYKIEPYHHQFWNGDYVQHLRPAYSFNV RMVSKRTRRSESGNKENLLGRYLSDGATNIQLRGPEYYNIMPVWEWDKIPGITSRDYLTD RPLTKLWGEQGSNDFAGGVSDGVYGASAYALDYDSLQAKKAWFFFDKEIVCLGAGINSNA PENITTTLNQSWLNGPVISTAGKTGRGKITTFKAQGQFWLLHDAIGYYFPEGANLSLSTQ SQKGNWFHINNSHSKDEVSGDVFKLWINHGARPENAQYAYIVLPGINKPEEIKKYNGTAP KVLANTNQLQAVYHQQLDMVQAIFYTAGKLSVAGIEIETDKPCAVLIKHINGKQVIWAAD PLQKEKTAVLSIRDLKTGKTNRVKIDFPQQEFAGATVELK
- Number of residues
- 700
- Molecular Weight
- 79693.285
- Theoretical pI
- 9.19
- GO Classification
- Functionscarbohydrate binding / chondroitin AC lyase activity / metal ion bindingProcessescarbohydrate metabolic processComponentsextracellular region
- General Function
- Metal ion binding
- Specific Function
- Not Available
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0010993|Chondroitinase-AC (cslA) ATGAAGAAATTATTTGTAACCTGTATAGTCTTTTTCTCTATTTTAAGTCCTGCTCTGCTT ATTGCACAGCAGACCGGTACTGCAGAACTGATTATGAAGCGGGTGATGCTGGACCTTAAA AAGCCTTTGCGCAATATGGATAAGGTGGCGGAAAAGAACCTGAATACGCTGCAGCCTGAC GGTAGCTGGAAGGATGTGCCTTATAAAGATGATGCCATGACCAATTGGTTGCCAAACAAC CACCTGCTACAATTGGAAACTATTATACAGGCTTATATTGAAAAAGATAGTCACTATTAT GGCGACGATAAAGTGTTTGACCAGATTTCCAAAGCTTTTAAGTATTGGTATGACAGCGAC CCGAAAAGCCGCAACTGGTGGCACAATGAAATTGCCACTCCGCAGGCCCTTGGTGAAATG CTGATCCTGATGCGTTACGGTAAAAAGCCGCTTGATGAAGCATTGGTGCATAAATTGACC GAAAGAATGAAGCGGGGCGAACCGGAGAAGAAAACGGGGGCCAACAAAACAGATATCGCC CTGCATTACTTTTATCGTGCTTTGTTAACGTCTGATGAGGCTTTGCTTTCCTTCGCCGTA AAAGAATTGTTTTATCCCGTACAGTTTGTACACTATGAGGAAGGCCTGCAATACGATTAT TCCTACCTGCAGCACGGTCCGCAATTACAGATATCGAGCTACGGTGCCGTATTTATTACC GGGGTACTGAAACTTGCCAATTACGTTAGGGATACCCCTTATGCTTTAAGTACCGAGAAA CTGGCTATATTTTCAAAGTATTACCGCGACAGTTATCTGAAAGCTATCCGTGGAAGTTAT ATGGATTTTAACGTAGAAGGCCGCGGAGTAAGCCGGCCAGACATTCTAAATAAAAAGGCA GAAAAAAAGAGGTTGCTGGTGGCGAAGATGATCGATCTTAAGCATACTGAAGAATGGGCT GATGCGATAGCCAGGACAGATAGCACAGTTGCGGCCGGCTATAAGATTGAGCCCTATCAC CATCAGTTCTGGAATGGTGATTATGTGCAACATTTAAGACCTGCCTATTCTTTTAATGTT CGTATGGTGAGTAAGCGGACCCGACGCAGTGAATCCGGCAATAAAGAAAACCTGCTGGGC AGGTATTTATCTGATGGGGCTACTAACATACAATTGCGCGGACCAGAATACTATAACATT ATGCCGGTATGGGAATGGGACAAGATTCCTGGCATAACCAGCCGTGATTATTTAACCGAC AGACCTTTGACGAAGCTTTGGGGAGAGCAGGGGAGCAATGACTTTGCAGGAGGGGTGTCT GATGGTGTATACGGGGCCAGTGCCTACGCATTGGATTACGATAGCTTACAGGCAAAGAAA GCCTGGTTCTTTTTTGACAAAGAGATTGTATGTCTTGGTGCCGGTATCAACAGCAATGCC CCTGAAAACATTACCACTACCCTTAACCAGAGCTGGTTAAATGGCCCGGTTATAAGTACT GCAGGTAAAACCGGCCGGGGTAAAATAACAACGTTTAAAGCACAGGGACAGTTCTGGTTG TTGCACGATGCGATTGGTTATTACTTTCCTGAAGGGGCCAACCTTAGTCTGAGTACCCAG TCGCAAAAAGGCAATTGGTTCCACATCAACAATTCACATTCAAAAGATGAAGTTTCTGGT GATGTATTTAAGCTTTGGATCAACCATGGTGCCAGGCCAGAAAATGCGCAGTATGCTTAT ATCGTTTTGCCGGGAATAAACAAGCCGGAAGAAATTAAAAAATATAATGGAACGGCACCG AAAGTCCTTGCCAATACCAACCAGCTGCAGGCAGTTTATCATCAGCAGTTAGATATGGTA CAGGCTATCTTCTATACAGCTGGAAAATTAAGCGTAGCGGGCATAGAAATTGAAACAGAT AAGCCATGTGCAGTGCTGATCAAGCACATCAATGGCAAGCAGGTAATTTGGGCTGCCGAT CCATTGCAAAAAGAAAAGACTGCAGTGTTGAGCATCAGGGATTTAAAAACAGGAAAAACA AATCGGGTAAAAATTGATTTTCCGCAACAGGAATTTGCAGGTGCAACGGTTGAACTGAAA TAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q59288 UniProtKB Entry Name CSLA_PEDHD GenBank Protein ID 1002525 GenBank Gene ID U27583 - General References
- Tkalec AL, Fink D, Blain F, Zhang-Sun G, Laliberte M, Bennett DC, Gu K, Zimmermann JJ, Su H: Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum. Appl Environ Microbiol. 2000 Jan;66(1):29-35. [Article]
- Han C, Spring S, Lapidus A, Del Rio TG, Tice H, Copeland A, Cheng JF, Lucas S, Chen F, Nolan M, Bruce D, Goodwin L, Pitluck S, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CC, Saunders E, Chertkov O, Brettin T, Goker M, Rohde M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Detter JC: Complete genome sequence of Pedobacter heparinus type strain (HIM 762-3). Stand Genomic Sci. 2009 Jul 20;1(1):54-62. doi: 10.4056/sigs.22138. [Article]
- Fethiere J, Eggimann B, Cygler M: Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes. J Mol Biol. 1999 May 14;288(4):635-47. [Article]
- Huang W, Boju L, Tkalec L, Su H, Yang HO, Gunay NS, Linhardt RJ, Kim YS, Matte A, Cygler M: Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis. Biochemistry. 2001 Feb 27;40(8):2359-72. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB01872 2-deoxy-2-acetamido-beta-D-galactose-4-sulfate experimental unknown Details DB02186 N-acetyl-beta-D-galactosamine 6-sulfate experimental unknown Details DB02305 4,5-Dehydro-D-Glucuronic Acid experimental unknown Details DB02945 alpha-L-iduronic acid experimental unknown Details DB03156 beta-D-glucuronic acid experimental unknown Details DB03389 alpha-D-Xylopyranose experimental unknown Details DB03569 4,5-Dehydro-L-Iduronic Acid experimental unknown Details DB03863 2-O-Methyl-beta-L-fucopyranose experimental unknown Details DB03879 alpha-L-methyl-fucose experimental unknown Details