Protein RecA
Details
- Name
- Protein RecA
- Synonyms
- Recombinase A
- Gene Name
- recA
- Organism
- Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
- Amino acid sequence
>lcl|BSEQ0016359|Protein RecA MAQQAPDREKALELAMAQIDKNFGKGSVMRLGEEVRQPISVIPTGSISLDVALGIGGLPR GRVIEIYGPESSGKTTVALHAVANAQAAGGIAAFIDAEHALDPEYAKKLGVDTDSLLVSQ PDTGEQALEIADMLVRSGALDIIVIDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKM TGALNNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRLDVRRIETLKDGTDAV GNRTRVKVVKNKVSPPFKQAEFDILYGQGISREGSLIDMGVEHGFIRKSGSWFTYEGEQL GQGKENARKFLLENTDVANEIEKKIKEKLGIGAVVTAEADDVLPAPVDF
- Number of residues
- 349
- Molecular Weight
- 37300.27
- Theoretical pI
- 5.05
- GO Classification
- FunctionsATP binding / damaged DNA binding / DNA-dependent ATPase activity / single-stranded DNA bindingProcessesDNA recombination / DNA repair / SOS responseComponentscytoplasm
- General Function
- Single-stranded dna binding
- Specific Function
- Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.
- Pfam Domain Function
- RecA (PF00154)
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0016360|Protein RecA (recA) ATGGCGCAGCAGGCCCCAGATCGCGAAAAGGCCCTCGAACTGGCGATGGCCCAGATCGAC AAGAATTTCGGCAAAGGCTCGGTGATGCGCCTCGGCGAAGAGGTGCGCCAGCCGATCTCG GTGATCCCCACCGGCTCCATCTCGCTGGATGTGGCGCTCGGCATCGGCGGCCTGCCGCGG GGCCGCGTCATCGAGATCTACGGCCCGGAGTCCTCGGGTAAGACCACCGTGGCCCTGCAT GCCGTCGCCAACGCGCAGGCCGCGGGCGGTATCGCGGCGTTCATCGACGCCGAGCACGCG CTGGATCCCGAGTACGCCAAGAAGCTGGGTGTGGACACCGACTCGCTGCTGGTGTCGCAG CCCGACACCGGTGAGCAGGCGCTGGAGATCGCCGACATGCTGGTGCGGTCCGGCGCGCTG GACATCATCGTCATCGACTCGGTCGCCGCCCTGGTGCCGCGCGCCGAGATCGAGGGCGAG ATGGGTGACAGCCACGTCGGCCTGCAGGCCCGCCTGATGAGCCAGGCGCTGCGCAAGATG ACCGGCGCGTTGAACAACTCGGGCACCACCGCGATCTTCATCAACCAGCTCCGCGAGAAG ATCGGTGTGATGTTCGGCTCGCCCGAGACCACCACGGGCGGTAAGGCACTGAAGTTCTAC GCCTCGGTCCGCCTCGACGTGCGCCGTATCGAGACGCTCAAGGACGGCACCGACGCGGTC GGTAACCGCACCCGCGTCAAGGTCGTCAAGAACAAGGTCTCCCCGCCGTTCAAGCAGGCC GAGTTCGACATCCTCTACGGCCAGGGCATCAGCCGCGAGGGTTCGCTCATCGACATGGGC GTCGAGCACGGCTTCATCCGCAAGTCCGGGTCCTGGTTCACCTACGAGGGTGAGCAGCTG GGCCAGGGCAAGGAGAACGCCCGCAAGTTCCTGCTGGAGAACACCGACGTCGCCAACGAG ATCGAGAAGAAGATCAAAGAGAAGCTCGGTATCGGCGCCGTCGTGACCGCTGAAGCCGAT GACGTCCTCCCGGCCCCGGTTGACTTCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q59560 UniProtKB Entry Name RECA_MYCS2 GenBank Protein ID 1430892 GenBank Gene ID X99208 - General References
- Papavinasasundaram KG, Colston MJ, Davis EO: Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA function. Mol Microbiol. 1998 Nov;30(3):525-34. [Article]
- Deshayes C, Perrodou E, Gallien S, Euphrasie D, Schaeffer C, Van-Dorsselaer A, Poch O, Lecompte O, Reyrat JM: Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors? Genome Biol. 2007;8(2):R20. [Article]
- Gallien S, Perrodou E, Carapito C, Deshayes C, Reyrat JM, Van Dorsselaer A, Poch O, Schaeffer C, Lecompte O: Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol. Genome Res. 2009 Jan;19(1):128-35. doi: 10.1101/gr.081901.108. Epub 2008 Oct 27. [Article]
- Pitcher RS, Green AJ, Brzostek A, Korycka-Machala M, Dziadek J, Doherty AJ: NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation. DNA Repair (Amst). 2007 Sep 1;6(9):1271-6. Epub 2007 Mar 13. [Article]
- Stephanou NC, Gao F, Bongiorno P, Ehrt S, Schnappinger D, Shuman S, Glickman MS: Mycobacterial nonhomologous end joining mediates mutagenic repair of chromosomal double-strand DNA breaks. J Bacteriol. 2007 Jul;189(14):5237-46. Epub 2007 May 11. [Article]
- Gupta R, Barkan D, Redelman-Sidi G, Shuman S, Glickman MS: Mycobacteria exploit three genetically distinct DNA double-strand break repair pathways. Mol Microbiol. 2011 Jan;79(2):316-30. doi: 10.1111/j.1365-2958.2010.07463.x. Epub 2010 Nov 24. [Article]
- Datta S, Krishna R, Ganesh N, Chandra NR, Muniyappa K, Vijayan M: Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexes. J Bacteriol. 2003 Jul;185(14):4280-4. [Article]
- Krishna R, Prabu JR, Manjunath GP, Datta S, Chandra NR, Muniyappa K, Vijayan M: Snapshots of RecA protein involving movement of the C-domain and different conformations of the DNA-binding loops: crystallographic and comparative analysis of 11 structures of Mycobacterium smegmatis RecA. J Mol Biol. 2007 Apr 6;367(4):1130-44. Epub 2007 Jan 26. [Article]
- Prabu JR, Manjunath GP, Chandra NR, Muniyappa K, Vijayan M: Functionally important movements in RecA molecules and filaments: studies involving mutation and environmental changes. Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1146-57. doi: 10.1107/S0907444908028448. Epub 2008 Oct 18. [Article]