Anoctamin-1

Details

Name

Anoctamin-1

Synonyms

• Discovered on gastrointestinal stromal tumors protein 1
• DOG1
• Oral cancer overexpressed protein 2
• ORAOV2
• TAOS2
• TMEM16A
• Transmembrane protein 16A
• Tumor-amplified and overexpressed sequence 2

Gene Name

ANO1

Organism

Humans

Amino acid sequence

>lcl|BSEQ0008597|Anoctamin-1
MRVNEKYSTLPAEDRSVHIINICAIEDIGYLPSEGTLLNSLSVDPDAECKYGLYFRDGRR
KVDYILVYHHKRPSGNRTLVRRVQHSDTPSGARSVKQDHPLPGKGASLDAGSGEPPMDYH
EDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHAPWNVLCREAEFLKLKMPTKK
MYHINETRGLLKKINSVLQKITDPIQPKVAEHRPQTMKRLSYPFSREKQHLFDLSDKDSF
FDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYAAAYPLHDGDYNGENVEFNDRKL
LYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAWLGVYTQMLIPASIVGIIVFLYGCATM
DENIPSMEMCDQRHNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWA
ATFMEHWKRKQMRLNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLKKESRNKEKRRHI
PEESTNKWKQRVKTAMAGVKLTDKVKLTWRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIY
RISMAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEK
SFEERLIFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMEL
CIQLSIIMLGKQLIQNNLFEIGIPKMKKLIRYLKLKQQSPPDHEECVKRKQRYEVDYNLE
PFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRA
KDIGIWYNILRGIGKLAVIINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNV
SDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYDISKDFWAVLAARLAFVIVFQNL
VMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQDKQQLLETWMEKERQKDEPPC
NHHNTKACPDSLGSPAPSHAYHGGVL

Number of residues

986

Molecular Weight

114077.215

Theoretical pI

Not Available

GO Classification

Functions

calcium activated cation channel activity / chloride channel activity / intracellular calcium activated chloride channel activity / iodide transmembrane transporter activity / voltage-gated chloride channel activity

Processes

cation transmembrane transport / cation transport / cellular response to heat / chloride transmembrane transport / chloride transport / detection of temperature stimulus involved in sensory perception of pain / iodide transport / ion transmembrane transport / multicellular organismal development / phospholipase C-activating G-protein coupled receptor signaling pathway / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of anion transmembrane transport / regulation of membrane potential / transmembrane transport

Components

apical plasma membrane / chloride channel complex / cytoplasm / external side of plasma membrane / extracellular exosome / plasma membrane

General Function

Voltage-gated chloride channel activity

Specific Function

Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.

Pfam Domain Function

• Anoct_dimer (PF16178)
• Anoctamin (PF04547)

Transmembrane Regions

334-354 403-423 520-540 566-586 607-627 732-752 792-812 883-903

Cellular Location

Cell membrane

Gene sequence

>lcl|BSEQ0017524|Anoctamin-1 (ANO1)
ATGAGGGTCAACGAGAAGTACTCGACGCTCCCGGCCGAGGACCGCAGCGTCCACATCATC
AACATCTGCGCCATCGAGGACATCGGCTACCTGCCGTCCGAGGGCACGCTGCTGAACTCC
TTATCTGTGGACCCTGATGCCGAGTGCAAGTATGGCCTGTACTTCAGGGACGGCCGGCGC
AAGGTGGACTACATCCTGGTGTACCATCACAAGAGGCCCTCGGGCAACCGGACCCTGGTC
AGGAGGGTGCAGCACAGCGACACCCCCTCTGGGGCTCGCAGCGTCAAGCAGGACCACCCC
CTGCCGGGCAAGGGGGCGTCGCTGGATGCAGGCTCGGGGGAGCCCCCGATGGACTACCAC
GAGGATGACAAGCGCTTCCGCAGGGAGGAGTACGAGGGCAACCTCCTGGAGGCGGGCCTG
GAGCTGGAGCGGGACGAGGACACTAAAATCCACGGAGTCGGGTTTGTGAAAATCCATGCC
CCCTGGAACGTGCTGTGCAGAGAGGCCGAGTTTCTGAAACTGAAGATGCCGACGAAGAAG
ATGTACCACATTAATGAGACCCGTGGCCTCCTGAAAAAAATCAACTCTGTGCTCCAGAAA
ATCACAGATCCCATCCAGCCCAAAGTGGCTGAGCACAGGCCCCAGACCATGAAGAGACTC
TCCTATCCCTTCTCCCGGGAGAAGCAGCATCTATTTGACTTGTCTGATAAGGATTCCTTT
TTCGACAGCAAAACCCGGAGCACGATTGTCTATGAGATCTTGAAGAGAACGACGTGTACA
AAGGCCAAGTACAGCATGGGCATCACGAGCCTGCTGGCCAATGGTGTGTACGCGGCTGCA
TACCCACTGCACGATGGAGACTACAACGGTGAAAACGTCGAGTTCAACGACAGAAAACTC
CTGTACGAAGAGTGGGCACGCTATGGAGTTTTCTATAAGTACCAGCCCATCGACCTGGTC
AGGAAGTATTTTGGGGAGAAGATCGGCCTGTACTTCGCCTGGCTGGGCGTGTACACCCAG
ATGCTCATCCCTGCCTCCATCGTGGGAATCATTGTCTTCCTGTACGGATGCGCCACCATG
GATGAAAACATCCCCAGCATGGAGATGTGTGACCAGAGACACAATATCACCATGTGCCCG
CTTTGCGACAAGACCTGCAGCTACTGGAAGATGAGCTCAGCCTGCGCCACGGCCCGCGCC
AGCCACCTCTTCGACAACCCCGCCACGGTCTTCTTCTCTGTCTTCATGGCCCTCTGGGCT
GCCACCTTCATGGAGCACTGGAAGCGGAAACAGATGCGACTCAACTACCGCTGGGACCTC
ACGGGCTTTGAAGAGGAAGAGGAGGCTGTCAAGGATCATCCTAGAGCTGAATACGAAGCC
AGAGTCTTGGAGAAGTCTCTGAAGAAAGAGTCCAGAAACAAAGAGAAGCGCCGGCATATT
CCAGAGGAGTCAACAAACAAATGGAAGCAGAGGGTTAAGACAGCCATGGCGGGGGTGAAA
TTGACTGACAAAGTGAAGCTGACATGGAGAGATCGGTTCCCAGCCTACCTCACTAACTTG
GTCTCCATCATCTTCATGATTGCAGTGACGTTTGCCATCGTCCTCGGCGTCATCATCTAC
AGAATCTCCATGGCCGCCGCCTTGGCCATGAACTCCTCCCCCTCCGTGCGGTCCAACATC
CGGGTCACAGTCACAGCCACCGCAGTCATCATCAACCTAGTGGTCATCATCCTCCTGGAC
GAGGTGTATGGCTGCATAGCCCGATGGCTCACCAAGATCGAGGTCCCAAAGACGGAGAAA
AGCTTTGAGGAGAGGCTGATCTTCAAGGCTTTCCTGCTGAAGTTTGTGAATTCCTACACC
CCCATCTTTTACGTGGCGTTCTTCAAAGGCCGGTTTGTTGGACGCCCGGGCGACTACGTG
TACATTTTCCGTTCCTTCCGAATGGAAGAGTGTGCGCCAGGGGGCTGCCTGATGGAGCTA
TGCATCCAGCTCAGCATCATCATGCTGGGGAAACAGCTGATCCAGAACAACCTGTTCGAG
ATCGGCATCCCGAAGATGAAGAAGCTCATCCGCTACCTGAAGCTGAAGCAGCAGAGCCCC
CCTGACCACGAGGAGTGTGTGAAGAGGAAACAGCGGTACGAGGTGGATTACAACCTGGAG
CCCTTCGCGGGCCTCACCCCAGAGTACATGGAAATGATCATCCAGTTTGGCTTCGTCACC
CTGTTTGTCGCCTCCTTCCCCCTGGCCCCACTGTTTGCGCTGCTGAACAACATCATCGAG
ATCCGCCTGGACGCCAAAAAGTTTGTCACTGAGCTCCGAAGGCCGGTAGCTGTCAGAGCC
AAAGACATCGGAATCTGGTACAATATCCTCAGAGGCATTGGGAAGCTTGCTGTCATCATC
AATGCCTTCGTGATCTCCTTCACGTCTGACTTCATCCCGCGCCTGGTGTACCTCTACATG
TACAGTAAGAACGGGACCATGCACGGCTTCGTCAACCACACCCTCTCCTCCTTCAACGTC
AGTGACTTCCAGAACGGCACGGCCCCCAATGACCCCCTGGACCTGGGCTACGAGGTGCAG
ATCTGCAGGTATAAAGACTACCGAGAGCCGCCGTGGTCGGAAAACAAGTACGACATCTCC
AAGGACTTCTGGGCCGTCCTGGCAGCCCGGCTGGCGTTTGTCATCGTCTTCCAGAACCTG
GTCATGTTCATGAGCGACTTTGTGGACTGGGTCATCCCGGACATCCCCAAGGACATCAGC
CAGCAGATCCACAAGGAGAAGGTGCTCATGGTGGAGCTGTTCATGCGGGAGGAGCAAGAC
AAGCAGCAGCTGCTGGAAACCTGGATGGAGAAGGAGCGGCAGAAGGACGAGCCGCCGTGC
AACCACCACAACACCAAAGCCTGCCCAGACAGCCTCGGCAGCCCAGCCCCCAGCCATGCC
TACCACGGGGGCGTCCTGTAG

Chromosome Location

11

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	Q5XXA6
UniProtKB Entry Name	ANO1_HUMAN
HGNC ID	HGNC:21625

General References

1. Huang X, Godfrey TE, Gooding WE, McCarty KS Jr, Gollin SM: Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma. Genes Chromosomes Cancer. 2006 Nov;45(11):1058-69. [Article]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [Article]
3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
4. Katoh M, Katoh M: FLJ10261 gene, located within the CCND1-EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int J Oncol. 2003 Jun;22(6):1375-81. [Article]
5. West RB, Corless CL, Chen X, Rubin BP, Subramanian S, Montgomery K, Zhu S, Ball CA, Nielsen TO, Patel R, Goldblum JR, Brown PO, Heinrich MC, van de Rijn M: The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status. Am J Pathol. 2004 Jul;165(1):107-13. [Article]
6. Carles A, Millon R, Cromer A, Ganguli G, Lemaire F, Young J, Wasylyk C, Muller D, Schultz I, Rabouel Y, Dembele D, Zhao C, Marchal P, Ducray C, Bracco L, Abecassis J, Poch O, Wasylyk B: Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display. Oncogene. 2006 Mar 16;25(12):1821-31. [Article]
7. Schreiber R, Uliyakina I, Kongsuphol P, Warth R, Mirza M, Martins JR, Kunzelmann K: Expression and function of epithelial anoctamins. J Biol Chem. 2010 Mar 5;285(10):7838-45. doi: 10.1074/jbc.M109.065367. Epub 2010 Jan 7. [Article]
8. Ferrera L, Caputo A, Galietta LJ: TMEM16A protein: a new identity for Ca(2+)-dependent Cl(-) channels. Physiology (Bethesda). 2010 Dec;25(6):357-63. doi: 10.1152/physiol.00030.2010. [Article]
9. Duran C, Hartzell HC: Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels? Acta Pharmacol Sin. 2011 Jun;32(6):685-92. doi: 10.1038/aps.2011.48. [Article]
10. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
11. Ousingsawat J, Kongsuphol P, Schreiber R, Kunzelmann K: CFTR and TMEM16A are separate but functionally related Cl- channels. Cell Physiol Biochem. 2011;28(4):715-24. doi: 10.1159/000335765. Epub 2011 Dec 14. [Article]
12. Sheridan JT, Worthington EN, Yu K, Gabriel SE, Hartzell HC, Tarran R: Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A. J Biol Chem. 2011 Jan 14;286(2):1381-8. doi: 10.1074/jbc.M110.174847. Epub 2010 Nov 5. [Article]
13. Kunzelmann K, Tian Y, Martins JR, Faria D, Kongsuphol P, Ousingsawat J, Thevenod F, Roussa E, Rock J, Schreiber R: Anoctamins. Pflugers Arch. 2011 Aug;462(2):195-208. doi: 10.1007/s00424-011-0975-9. Epub 2011 May 21. [Article]
14. Winpenny JP, Gray MA: The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age. Exp Physiol. 2012 Feb;97(2):175-6. doi: 10.1113/expphysiol.2011.058214. [Article]
15. Scudieri P, Sondo E, Ferrera L, Galietta LJ: The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels. Exp Physiol. 2012 Feb;97(2):177-83. doi: 10.1113/expphysiol.2011.058198. Epub 2011 Oct 7. [Article]
16. Kunzelmann K, Schreiber R, Kmit A, Jantarajit W, Martins JR, Faria D, Kongsuphol P, Ousingsawat J, Tian Y: Expression and function of epithelial anoctamins. Exp Physiol. 2012 Feb;97(2):184-92. doi: 10.1113/expphysiol.2011.058206. Epub 2011 Sep 9. [Article]
17. Tian Y, Schreiber R, Kunzelmann K: Anoctamins are a family of Ca2+-activated Cl- channels. J Cell Sci. 2012 Nov 1;125(Pt 21):4991-8. doi: 10.1242/jcs.109553. Epub 2012 Sep 3. [Article]
18. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB04941	Crofelemer	approved	yes	antagonist	Details