CCA-adding enzyme
Details
- Name
- CCA-adding enzyme
- Synonyms
- 2.7.7.72
- CCA tRNA nucleotidyltransferase
- tRNA adenylyl-/cytidylyl- transferase
- tRNA CCA-pyrophosphorylase
- tRNA nucleotidyltransferase
- tRNA-NT
- Gene Name
- cca
- Organism
- Geobacillus stearothermophilus
- Amino acid sequence
>lcl|BSEQ0022032|CCA-adding enzyme MKPPFQEALGIIQQLKQHGYDAYFVGGAVRDLLLGRPIGDVDIATSALPEDVMAIFPKTI DVGSKHGTVVVVHKGKAYEVTTFKTDGDYEDYRRPESVTFVRSLEEDLKRRDFTMNAIAM DEYGTIIDPFGGREAIRRRIIRTVGEAEKRFREDALRMMRAVRFVSELGFALAPDTEQAI VQNAPLLAHISVERMTMEMEKLLGGPFAARALPLLAETGLNAYLPGLAGKEKQLRLAAAY RWPWLAAREERWALLCHALGVQESRPFLRAWKLPNKVVDEAGAILTALADIPRPEAWTNE QLFSAGLERALSVETVRAAFTGAPPGPWHEKLRRRFASLPIKTKGELAVNGKDVIEWVGK PAGPWVKEALDAIWRAVVNGEVENEKERIYAWLMERNRTREKNC
- Number of residues
- 404
- Molecular Weight
- 45368.875
- Theoretical pI
- 8.61
- GO Classification
- FunctionsATP / ATP binding / CTP / magnesium ion binding / tRNA binding / tRNA cytidylyltransferase activityProcessesRNA repair / tRNA 3'-terminal CCA addition
- General Function
- Trna cytidylyltransferase activity
- Specific Function
- Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.
- Pfam Domain Function
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q7SIB1 UniProtKB Entry Name CCA_GEOSE - General References
- Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA: Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP. Cell. 2002 Dec 13;111(6):815-24. [Article]