Equilibrative nucleoside transporter 1

Details

Name
Equilibrative nucleoside transporter 1
Synonyms
  • ENT1
  • Equilibrative NBMPR-sensitive nucleoside transporter
  • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
  • Nucleoside transporter, es-type
  • Solute carrier family 29 member 1
Gene Name
SLC29A1
Organism
Humans
Amino acid sequence
>lcl|BSEQ0002395|Equilibrative nucleoside transporter 1
MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDA
QASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAIL
LVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQ
GLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKL
EGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIF
TITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRW
LPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPK
KVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV
Number of residues
456
Molecular Weight
50218.805
Theoretical pI
8.39
GO Classification
Functions
nucleoside transmembrane transporter activity
Processes
cellular response to glucose stimulus / cellular response to hypoxia / excitatory postsynaptic potential / lactation / nucleobase-containing compound metabolic process / nucleoside transmembrane transport / nucleoside transport / sleep / transmembrane transport / uridine transport
Components
apical plasma membrane / basolateral plasma membrane / integral component of plasma membrane / membrane / plasma membrane
General Function
Nucleoside transmembrane transporter activity
Specific Function
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Pfam Domain Function
Transmembrane Regions
13-29 83-107 112-130 139-157 175-199 207-227 292-311 324-342 360-378 394-413 432-452
Cellular Location
Basolateral cell membrane
Gene sequence
>lcl|BSEQ0019080|Equilibrative nucleoside transporter 1 (SLC29A1)
ATGACAACCAGTCACCAGCCTCAGGACAGATACAAAGCTGTCTGGCTTATCTTCTTCATG
CTGGGTCTGGGAACGCTGCTCCCGTGGAATTTTTTCATGACGGCCACTCAGTATTTCACA
AACCGCCTGGACATGTCCCAGAATGTGTCCTTGGTCACTGCTGAACTGAGCAAGGACGCC
CAGGCGTCAGCCGCCCCTGCAGCACCCTTGCCTGAGCGGAACTCTCTCAGTGCCATCTTC
AACAATGTCATGACCCTATGTGCCATGCTGCCCCTGCTGTTATTCACCTACCTCAACTCC
TTCCTGCATCAGAGGATCCCCCAGTCCGTACGGATCCTGGGCAGCCTGGTGGCCATCCTG
CTGGTGTTTCTGATCACTGCCATCCTGGTGAAGGTGCAGCTGGATGCTCTGCCCTTCTTT
GTCATCACCATGATCAAGATCGTGCTCATTAATTCATTTGGTGCCATCCTGCAGGGCAGC
CTGTTTGGTCTGGCTGGCCTTCTGCCTGCCAGCTACACGGCCCCCATCATGAGTGGCCAG
GGCCTAGCAGGCTTCTTTGCCTCCGTGGCCATGATCTGCGCTATTGCCAGTGGCTCGGAG
CTATCAGAAAGTGCCTTCGGCTACTTTATCACAGCCTGTGCTGTTATCATTTTGACCATC
ATCTGTTACCTGGGCCTGCCCCGCCTGGAATTCTACCGCTACTACCAGCAGCTCAAGCTT
GAAGGACCCGGGGAGCAGGAGACCAAGTTGGACCTCATTAGCAAAGGAGAGGAGCCAAGA
GCAGGCAAAGAGGAATCTGGAGTTTCAGTCTCCAACTCTCAGCCCACCAATGAAAGCCAC
TCTATCAAAGCCATCCTGAAAAATATCTCAGTCCTGGCTTTCTCTGTCTGCTTCATCTTC
ACTATCACCATTGGGATGTTTCCAGCCGTGACTGTTGAGGTCAAGTCCAGCATCGCAGGC
AGCAGCACCTGGGAACGTTACTTCATTCCTGTGTCCTGTTTCTTGACTTTCAATATCTTT
GACTGGTTGGGCCGGAGCCTCACAGCTGTATTCATGTGGCCTGGGAAGGACAGCCGCTGG
CTGCCAAGCCTGGTGCTGGCCCGGCTGGTGTTTGTGCCACTGCTGCTGCTGTGCAACATT
AAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGATGCCTGGTTCATCTTCTTCATG
GCTGCCTTTGCCTTCTCCAACGGCTACCTCGCCAGCCTCTGCATGTGCTTCGGGCCCAAG
AAAGTGAAGCCAGCTGAGGCAGAGACCGCAGGAGCCATCATGGCCTTCTTCCTGTGTCTG
GGTCTGGCACTGGGGGCTGTTTTCTCCTTCCTGTTCCGGGCAATTGTGTGA
Chromosome Location
6
Locus
6p21.2-p21.1
External Identifiers
ResourceLink
UniProtKB IDQ99808
UniProtKB Entry NameS29A1_HUMAN
GenBank Protein ID1845345
GenBank Gene IDU81375
GenAtlas IDSLC29A1
HGNC IDHGNC:11003
General References
  1. Griffiths M, Beaumont N, Yao SY, Sundaram M, Boumah CE, Davies A, Kwong FY, Coe I, Cass CE, Young JD, Baldwin SA: Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat Med. 1997 Jan;3(1):89-93. [PubMed:8986748]
  2. Lum PY, Ngo LY, Bakken AH, Unadkat JD: Human intestinal es nucleoside transporter: molecular characterization and nucleoside inhibitory profiles. Cancer Chemother Pharmacol. 2000;45(4):273-8. [PubMed:10755314]
  3. Sankar N, Machado J, Abdulla P, Hilliker AJ, Coe IR: Comparative genomic analysis of equilibrative nucleoside transporters suggests conserved protein structure despite limited sequence identity. Nucleic Acids Res. 2002 Oct 15;30(20):4339-50. [PubMed:12384580]
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Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB00197Troglitazoneapproved, investigational, withdrawnnoinhibitorDetails
DB00987Cytarabineapproved, investigationalunknownsubstrateDetails
DB01073FludarabineapprovedunknownsubstrateDetails
DB00441GemcitabineapprovedunknownsubstrateDetails
DB00811RibavirinapprovedunknownsubstrateDetails
DB00642Pemetrexedapproved, investigationalunknowninducerDetails
DB02745Uridineexperimental, investigationalunknownDetails
DB00943Zalcitabineapproved, investigationalunknownDetails
DB00900DidanosineapprovedunknownDetails
DB00544FluorouracilapprovedunknownsubstrateDetails
DB01033MercaptopurineapprovedunknownsubstrateDetails
DB00898EthanolapprovedunknownDetails
DB09061Cannabidiolapproved, investigationalunknowninhibitorDetails
DB09327Tegafur-uracilapproved, investigationalnosubstrateDetails
DB14011NabiximolsinvestigationalunknowninhibitorDetails
DB14009Medical Cannabisexperimental, investigationalunknowninhibitorDetails
DB12010Fostamatinibapproved, investigationalunknowninhibitorDetails
DB00432Trifluridineapproved, investigationalunknownsubstrateDetails