NADPH azoreductase
Details
- Name
- NADPH azoreductase
- Synonyms
- 1.7.1.6
- Gene Name
- azr
- Organism
- Bacillus sp. (strain OY1-2)
- Amino acid sequence
>lcl|BSEQ0010445|NADPH azoreductase MKLVVINGTPRKFGRTRVVAKYIADQFEGELYDLAIEELPLYNGEESQRDLEAVKKLKTL VKAADGVVLCTPEYHNAMSGALKNSLDYLSSSEFIHKPVALLAVAGGGKGGINALNSMHA SLAGVYANAIPKQVVLDGLHVQDGELGEDAKPLIHDVVKELKAYMSVYKEVKKQLGVE
- Number of residues
- 178
- Molecular Weight
- 19293.155
- Theoretical pI
- 6.68
- GO Classification
- Functionsazobenzene reductase activity
- General Function
- Azobenzene reductase activity
- Specific Function
- Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH as an electron donor for its activity. Compounds with paired naphthalene groups coupled with the azo group are good substrates, with the following preference order: Rocceline > Sumifix Black B > Solar Orange.
- Pfam Domain Function
- FMN_red (PF03358)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0001271|537 bp ATGAAACTAGTCGTTATTAACGGTACACCAAGAAAATTTGGTAGAACTCGCGTGGTGGCA AAATATATTGCGGATCAATTTGAAGGGGAATTATATGATTTAGCAATTGAGGAGTTACCT TTATACAATGGAGAAGAGTCGCAACGTGATTTAGAGGCAGTAAAAAAATTAAAAACGTTA GTGAAAGCTGCGGATGGGGTTGTATTATGTACACCAGAATATCATAATGCGATGAGCGGT GCGCTGAAAAACTCTTTAGATTACTTAAGTAGTAGTGAATTTATTCATAAACCAGTTGCA TTGTTAGCGGTTGCTGGTGGCGGTAAAGGTGGAATAAATGCATTAAACAGCATGCACGCG TCGCTAGCAGGTGTTTATGCAAATGCAATTCCAAAACAAGTTGTGCTTGATGGATTACAT GTGCAAGATGGTGAACTTGGGGAAGATGCAAAACCATTAATTCATGATGTAGTTAAAGAA TTGAAAGCATATATGAGCGTATATAAAGAGGTGAAAAAACAACTAGGAGTGGAGTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9FAW5 UniProtKB Entry Name AZRB_BACOY GenBank Protein ID 10281002 GenBank Gene ID AB032601 - General References
- Suzuki Y, Yoda T, Ruhul A, Sugiura W: Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. 2001 Mar 23;276(12):9059-65. Epub 2000 Dec 27. [Article]