Cocaine esterase

Details

Name
Cocaine esterase
Synonyms
  • 3.1.1.84
Gene Name
cocE
Organism
Rhodococcus sp. (strain MB1 Bresler)
Amino acid sequence
>lcl|BSEQ0010990|Cocaine esterase
MVDGNYSVASNVMVPMRDGVRLAVDLYRPDADGPVPVLLVRNPYDKFDVFAWSTQSTNWL
EFVRDGYAVVIQDTRGLFASEGEFVPHVDDEADAEDTLSWILEQAWCDGNVGMFGVSYLG
VTQWQAAVSGVGGLKAIAPSMASADLYRAPWYGPGGALSVEALLGWSALIGTGLITSRSD
ARPEDAADFVQLAAILNDVAGAASVTPLAEQPLLGRLIPWVIDQVVDHPDNDESWQSISL
FERLGGLATPALITAGWYDGFVGESLRTFVAVKDNADARLVVGPWSHSNLTGRNADRKFG
IAATYPIQEATTMHKAFFDRHLRGETDALAGVPKVRLFVMGIDEWRDETDWPLPDTAYTP
FYLGGSGAANTSTGGGTLSTSISGTESADTYLYDPADPVPSLGGTLLFHNGDNGPADQRP
IHDRDDVLCYSTEVLTDPVEVTGTVSARLFVSSSAVDTDFTAKLVDVFPDGRAIALCDGI
VRMRYRETLVNPTLIEAGEIYEVAIDMLATSNVFLPGHRIMVQVSSSNFPKYDRNSNTGG
VIAREQLEEMCTAVNRIHRGPEHPSHIVLPIIKR
Number of residues
574
Molecular Weight
62131.125
Theoretical pI
4.31
GO Classification
Functions
carboxylic ester hydrolase activity / dipeptidyl-peptidase activity
Processes
cocaine catabolic process
Components
cytoplasm
General Function
Dipeptidyl-peptidase activity
Specific Function
Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants. Also efficiently hydrolyzes cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. Is able to prevent cocaine-induced convulsions and lethality in rat.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0002804|1725 bp
ATGGTGGACGGGAATTACAGTGTTGCCTCGAACGTGATGGTTCCGATGCGTGATGGGGTG
CGTCTGGCGGTCGACCTGTACCGACCAGATGCTGATGGACCTGTTCCGGTCCTGCTGGTT
CGCAACCCATACGACAAGTTCGACGTGTTCGCGTGGTCGACGCAGTCGACAAACTGGCTT
GAGTTCGTGCGTGATGGCTATGCCGTGGTCATTCAAGACACGCGTGGCTTGTTCGCATCG
GAAGGTGAGTTCGTCCCGCACGTTGACGACGAAGCTGACGCCGAGGATACGTTGAGCTGG
ATTCTGGAACAAGCGTGGTGCGACGGCAATGTGGGCATGTTCGGCGTTTCGTACTTGGGT
GTGACCCAGTGGCAGGCCGCCGTATCCGGCGTTGGTGGGCTGAAGGCGATCGCGCCGTCC
ATGGCGTCGGCGGACTTGTACCGCGCCCCGTGGTACGGCCCTGGTGGTGCGCTTTCAGTC
GAGGCGCTGTTGGGCTGGTCAGCTCTCATAGGTACTGGGCTCATCACGTCGAGGTCTGAC
GCCCGGCCCGAAGACGCAGCCGACTTCGTCCAACTCGCAGCAATTCTCAATGACGTCGCT
GGCGCGGCGTCGGTCACGCCCCTGGCCGAGCAACCGCTTCTGGGCCGACTGATTCCGTGG
GTGATCGATCAGGTTGTCGATCACCCCGACAACGATGAATCATGGCAGTCCATTAGCTTG
TTTGAACGACTCGGCGGGTTGGCAACACCGGCCTTGATCACGGCTGGGTGGTACGACGGG
TTCGTCGGCGAATCGTTGCGCACTTTCGTTGCGGTCAAGGACAATGCCGACGCACGTTTG
GTTGTCGGCCCTTGGAGTCACAGCAACCTCACTGGTCGGAATGCGGACCGGAAGTTCGGC
ATTGCCGCGACCTACCCGATTCAAGAAGCCACCACGATGCACAAGGCATTCTTCGACCGG
CACCTCCGCGGCGAGACCGATGCACTCGCAGGCGTCCCCAAAGTGCGGCTGTTCGTAATG
GGCATCGATGAGTGGCGTGACGAAACGGACTGGCCACTGCCGGACACGGCGTATACGCCC
TTCTATCTTGGAGGTAGCGGGGCTGCGAATACCTCCACGGGTGGTGGAACACTGTCGACG
TCGATTTCCGGAACTGAATCTGCTGACACCTACCTGTATGATCCGGCCGATCCCGTGCCT
TCGCTCGGGGGGACGCTGCTGTTCCACAACGGAGACAACGGACCCGCCGACCAACGTCCC
ATTCATGACCGGGACGACGTGTTGTGTTACAGCACTGAGGTATTGACCGACCCGGTGGAA
GTAACCGGCACCGTCTCCGCCCGGCTGTTCGTGTCGTCATCAGCGGTGGACACTGATTTC
ACCGCCAAACTTGTCGACGTATTTCCCGACGGTCGCGCGATCGCGCTGTGTGACGGGATC
GTGCGGATGCGGTACCGCGAGACGTTGGTCAATCCAACCTTGATCGAAGCGGGCGAAATC
TACGAGGTTGCTATCGACATGCTTGCAACCTCGAATGTATTCCTGCCAGGGCATCGCATC
ATGGTCCAAGTATCAAGTAGCAACTTCCCGAAATACGACCGCAATTCGAATACCGGCGGA
GTAATCGCACGGGAACAGCTCGAAGAGATGTGCACCGCCGTGAACCGCATTCACCGAGGA
CCTGAGCATCCCAGCCACATTGTGCTGCCGATTATCAAGCGATAG
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ9L9D7
UniProtKB Entry NameCOCE_RHOSM
GenBank Protein ID7229394
GenBank Gene IDAF173165
General References
  1. Bresler MM, Rosser SJ, Basran A, Bruce NC: Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1. Appl Environ Microbiol. 2000 Mar;66(3):904-8. [Article]
  2. Cooper ZD, Narasimhan D, Sunahara RK, Mierzejewski P, Jutkiewicz EM, Larsen NA, Wilson IA, Landry DW, Woods JH: Rapid and robust protection against cocaine-induced lethality in rats by the bacterial cocaine esterase. Mol Pharmacol. 2006 Dec;70(6):1885-91. Epub 2006 Sep 12. [Article]
  3. Larsen NA, Turner JM, Stevens J, Rosser SJ, Basran A, Lerner RA, Bruce NC, Wilson IA: Crystal structure of a bacterial cocaine esterase. Nat Struct Biol. 2002 Jan;9(1):17-21. [Article]
  4. Turner JM, Larsen NA, Basran A, Barbas CF 3rd, Bruce NC, Wilson IA, Lerner RA: Biochemical characterization and structural analysis of a highly proficient cocaine esterase. Biochemistry. 2002 Oct 15;41(41):12297-307. [Article]
  5. Narasimhan D, Nance MR, Gao D, Ko MC, Macdonald J, Tamburi P, Yoon D, Landry DM, Woods JH, Zhan CG, Tesmer JJ, Sunahara RK: Structural analysis of thermostabilizing mutations of cocaine esterase. Protein Eng Des Sel. 2010 Jul;23(7):537-47. doi: 10.1093/protein/gzq025. Epub 2010 Apr 30. [Article]
  6. Narasimhan D, Collins GT, Nance MR, Nichols J, Edwald E, Chan J, Ko MC, Woods JH, Tesmer JJ, Sunahara RK: Subunit stabilization and polyethylene glycolation of cocaine esterase improves in vivo residence time. Mol Pharmacol. 2011 Dec;80(6):1056-65. doi: 10.1124/mol.111.074997. Epub 2011 Sep 2. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB01795Phenylboronic acidexperimentalunknownDetails
DB03793Benzoic acidapproved, investigationalunknownproduct ofDetails