Ammonium transporter Rh type C

Details

Name

Ammonium transporter Rh type C

Synonyms

• C15orf6
• CDRC2
• PDRC2
• Rh family type C glycoprotein
• Rh glycoprotein kidney
• Rh type C glycoprotein
• Rhesus blood group family type C glycoprotein
• RHGK
• Tumor-related protein DRC2

Gene Name

RHCG

Organism

Humans

Amino acid sequence

>lcl|BSEQ0019496|Ammonium transporter Rh type C
MAWNTNLRWRLPLTCLLLQVIMVILFGVFVRYDFEADAHWWSERTHKNLSDMENEFYYRY
PSFQDVHVMVFVGFGFLMTFLQRYGFSAVGFNFLLAAFGIQWALLMQGWFHFLQDRYIVV
GVENLINADFCVASVCVAFGAVLGKVSPIQLLIMTFFQVTLFAVNEFILLNLLKVKDAGG
SMTIHTFGAYFGLTVTRILYRRNLEQSKERQNSVYQSDLFAMIGTLFLWMYWPSFNSAIS
YHGDSQHRAAINTYCSLAACVLTSVAISSALHKKGKLDMVHIQNATLAGGVAVGTAAEMM
LMPYGALIIGFVCGIISTLGFVYLTPFLESRLHIQDTCGINNLHGIPGIIGGIVGAVTAA
SASLEVYGKEGLVHSFDFQGFNGDWTARTQGKFQIYGLLVTLAMALMGGIIVGLILRLPF
WGQPSDENCFEDAVYWEMPEGNSTVYIPEDPTFKPSGPSVPSVPMVSPLPMASSVPLVP

Number of residues

479

Molecular Weight

53178.56

Theoretical pI

6.37

GO Classification

Functions

ammonium transmembrane transporter activity / ankyrin binding

Processes

amine transport / ammonium transport / cellular ion homeostasis / epithelial cell differentiation / homeostatic process / regulation of pH / transepithelial ammonium transport / transmembrane transport

Components

apical plasma membrane / basolateral plasma membrane / cytoplasmic vesicle / extracellular exosome / integral component of plasma membrane / plasma membrane

General Function

Ankyrin binding

Specific Function

Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.

Pfam Domain Function

• Ammonium_transp (PF00909)

Transmembrane Regions

10-30 61-81 86-106 124-144 149-169 178-200 219-239 251-271 282-302 304-324 346-366 395-415

Cellular Location

Apical cell membrane

Gene sequence

>lcl|BSEQ0019497|Ammonium transporter Rh type C (RHCG)
ATGGCCTGGAACACCAACCTCCGCTGGCGGCTGCCGCTCACCTGCCTGCTCCTGCAGGTG
ATTATGGTGATTCTCTTCGGGGTGTTCGTGCGCTACGACTTCGAGGCCGACGCCCACTGG
TGGTCAGAGAGGACGCACAAGAACTTGAGCGACATGGAGAACGAATTCTACTATCGCTAC
CCAAGCTTCCAGGACGTGCACGTGATGGTCTTCGTGGGCTTCGGCTTCCTCATGACTTTC
CTGCAGCGCTACGGCTTCAGCGCCGTGGGCTTCAACTTCCTGTTGGCAGCCTTCGGCATC
CAGTGGGCGCTGCTCATGCAGGGCTGGTTCCACTTCTTACAAGACCGCTACATCGTCGTG
GGCGTGGAGAACCTCATCAACGCTGACTTCTGCGTGGCCTCTGTCTGCGTGGCCTTTGGG
GCAGTTCTGGGTAAAGTCAGCCCCATTCAGCTGCTCATCATGACTTTCTTCCAAGTGACC
CTCTTCGCTGTGAATGAGTTCATTCTCCTTAACCTGCTAAAGGTGAAGGATGCAGGAGGC
TCCATGACCATCCACACATTTGGCGCCTACTTTGGGCTCACAGTGACCCGGATCCTCTAC
CGACGCAACCTAGAGCAGAGCAAGGAGAGACAGAATTCTGTGTACCAGTCGGACCTCTTT
GCCATGATTGGCACCCTCTTCCTGTGGATGTACTGGCCCAGCTTCAACTCAGCCATATCC
TACCATGGGGACAGCCAGCACCGAGCCGCCATCAACACCTACTGCTCCTTGGCAGCCTGC
GTGCTTACCTCGGTGGCAATATCCAGTGCCCTGCACAAGAAGGGCAAGCTGGACATGGTG
CACATCCAGAATGCCACGCTCGCAGGAGGGGTGGCCGTGGGTACCGCTGCTGAGATGATG
CTCATGCCTTACGGTGCCCTCATCATCGGCTTCGTCTGCGGCATCATCTCCACCCTGGGT
TTTGTATACCTGACCCCATTCCTGGAGTCCCGGCTGCACATCCAGGACACATGTGGCATT
AACAATCTGCATGGCATTCCTGGCATCATAGGCGGCATCGTGGGTGCTGTGACAGCGGCC
TCCGCCAGCCTTGAAGTCTATGGAAAAGAAGGGCTTGTCCATTCCTTTGACTTTCAAGGT
TTCAACGGGGACTGGACCGCAAGAACACAGGGAAAGTTCCAGATTTATGGTCTCTTGGTG
ACCCTGGCCATGGCCCTGATGGGTGGCATCATTGTGGGGCTCATTTTGAGATTACCATTC
TGGGGACAACCTTCAGATGAGAACTGCTTTGAGGATGCGGTCTACTGGGAGATGCCTGAA
GGGAACAGCACTGTCTACATCCCTGAGGACCCCACCTTCAAGCCCTCAGGACCCTCAGTA
CCCTCAGTACCCATGGTGTCCCCACTACCCATGGCTTCCTCGGTACCCTTGGTACCCTAG

Chromosome Location

15

Locus

15q25

External Identifiers

Resource	Link
UniProtKB ID	Q9UBD6
UniProtKB Entry Name	RHCG_HUMAN
GenBank Protein ID	6625732
GenBank Gene ID	AF193809
HGNC ID	HGNC:18140

General References

1. Liu Z, Chen Y, Mo R, Hui C, Cheng JF, Mohandas N, Huang CH: Characterization of human RhCG and mouse Rhcg as novel nonerythroid Rh glycoprotein homologues predominantly expressed in kidney and testis. J Biol Chem. 2000 Aug 18;275(33):25641-51. [Article]
2. Chen BS, Xu ZX, Xu X, Cai Y, Han YL, Wang J, Xia SH, Hu H, Wei F, Wu M, Wang MR: RhCG is downregulated in oesophageal squamous cell carcinomas, but expressed in multiple squamous epithelia. Eur J Cancer. 2002 Sep;38(14):1927-36. [Article]
3. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [Article]
4. Zody MC, Garber M, Sharpe T, Young SK, Rowen L, O'Neill K, Whittaker CA, Kamal M, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Kodira CD, Madan A, Qin S, Yang X, Abbasi N, Abouelleil A, Arachchi HM, Baradarani L, Birditt B, Bloom S, Bloom T, Borowsky ML, Burke J, Butler J, Cook A, DeArellano K, DeCaprio D, Dorris L 3rd, Dors M, Eichler EE, Engels R, Fahey J, Fleetwood P, Friedman C, Gearin G, Hall JL, Hensley G, Johnson E, Jones C, Kamat A, Kaur A, Locke DP, Madan A, Munson G, Jaffe DB, Lui A, Macdonald P, Mauceli E, Naylor JW, Nesbitt R, Nicol R, O'Leary SB, Ratcliffe A, Rounsley S, She X, Sneddon KM, Stewart S, Sougnez C, Stone SM, Topham K, Vincent D, Wang S, Zimmer AR, Birren BW, Hood L, Lander ES, Nusbaum C: Analysis of the DNA sequence and duplication history of human chromosome 15. Nature. 2006 Mar 30;440(7084):671-5. [Article]
5. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
6. Marini AM, Matassi G, Raynal V, Andre B, Cartron JP, Cherif-Zahar B: The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet. 2000 Nov;26(3):341-4. [Article]
7. Bakouh N, Benjelloun F, Hulin P, Brouillard F, Edelman A, Cherif-Zahar B, Planelles G: NH3 is involved in the NH4+ transport induced by the functional expression of the human Rh C glycoprotein. J Biol Chem. 2004 Apr 16;279(16):15975-83. Epub 2004 Feb 3. [Article]
8. Zidi-Yahiaoui N, Mouro-Chanteloup I, D'Ambrosio AM, Lopez C, Gane P, Le van Kim C, Cartron JP, Colin Y, Ripoche P: Human Rhesus B and Rhesus C glycoproteins: properties of facilitated ammonium transport in recombinant kidney cells. Biochem J. 2005 Oct 1;391(Pt 1):33-40. [Article]
9. Marini AM, Boeckstaens M, Benjelloun F, Cherif-Zahar B, Andre B: Structural involvement in substrate recognition of an essential aspartate residue conserved in Mep/Amt and Rh-type ammonium transporters. Curr Genet. 2006 Jun;49(6):364-74. Epub 2006 Feb 14. [Article]
10. Zidi-Yahiaoui N, Ripoche P, Le Van Kim C, Gane P, D'Ambrosio AM, Cartron JP, Colin Y, Mouro-Chanteloup I: Ammonium transport properties of HEK293 cells expressing RhCG mutants: preliminary analysis of structure/function by site-directed mutagenesis. Transfus Clin Biol. 2006 Mar-Apr;13(1-2):128-31. Epub 2006 Apr 3. [Article]
11. Gruswitz F, Chaudhary S, Ho JD, Schlessinger A, Pezeshki B, Ho CM, Sali A, Westhoff CM, Stroud RM: Function of human Rh based on structure of RhCG at 2.1 A. Proc Natl Acad Sci U S A. 2010 May 25;107(21):9638-43. doi: 10.1073/pnas.1003587107. Epub 2010 May 10. [Article]

Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB09326	Ammonia N-13	approved	unknown	transporter	Details