Limonene-1,2-epoxide hydrolase
Details
- Name
- Limonene-1,2-epoxide hydrolase
- Synonyms
- 3.3.2.8
- Gene Name
- limA
- Organism
- Rhodococcus erythropolis
- Amino acid sequence
>lcl|BSEQ0011337|Limonene-1,2-epoxide hydrolase MTSKIEQPRWASKDSAAGAASTPDEKIVLEFMDALTSNDAAKLIEYFAEDTMYQNMPLPP AYGRDAVEQTLAGLFTVMSIDAVETFHIGSSNGLVYTERVDVLRALPTGKSYNLSILGVF QLTEGKITGWRDYFDLREFEEAVDLPLRG
- Number of residues
- 149
- Molecular Weight
- 16520.47
- Theoretical pI
- 4.21
- GO Classification
- Functionslimonene-1,2-epoxide hydrolase activity
- General Function
- Limonene-1,2-epoxide hydrolase activity
- Specific Function
- Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.
- Pfam Domain Function
- LEH (PF07858)
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0003694|450 bp ATGACATCAAAGATCGAACAACCTCGCTGGGCGTCCAAGGACAGTGCCGCCGGCGCTGCC TCGACTCCGGACGAAAAGATCGTTCTGGAGTTCATGGACGCACTGACCAGTAATGATGCT GCAAAACTCATTGAGTACTTTGCAGAAGACACGATGTACCAGAACATGCCACTCCCCCCT GCATACGGCCGCGACGCCGTCGAGCAAACTCTGGCTGGCCTGTTCACCGTCATGAGCATC GATGCGGTGGAGACGTTCCATATCGGCTCGAGTAACGGACTTGTGTACACCGAACGTGTC GATGTCCTACGCGCACTACCCACCGGCAAGAGCTACAACCTGTCAATCCTCGGAGTCTTC CAGCTCACCGAGGGCAAGATTACGGGTTGGCGTGACTACTTCGATCTGCGCGAATTCGAA GAAGCTGTCGACCTTCCCCTCCGCGGCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID Q9ZAG3 UniProtKB Entry Name LIMA_RHOER GenBank Protein ID 4160334 GenBank Gene ID Y18005 - General References
- Barbirato F, Verdoes JC, de Bont JA, van der Werf MJ: The Rhodococcus erythropolis DCL14 limonene-1,2-epoxide hydrolase gene encodes an enzyme belonging to a novel class of epoxide hydrolases. FEBS Lett. 1998 Nov 6;438(3):293-6. [Article]
- van der Werf MJ, Overkamp KM, de Bont JA: Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14 belongs to a novel class of epoxide hydrolases. J Bacteriol. 1998 Oct;180(19):5052-7. [Article]
- Arand M, Hallberg BM, Zou J, Bergfors T, Oesch F, van der Werf MJ, de Bont JA, Jones TA, Mowbray SL: Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site. EMBO J. 2003 Jun 2;22(11):2583-92. [Article]