Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.
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Fouet A, Arnaud M, Klier A, Rapoport G
Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.
Biochem Biophys Res Commun. 1984 Mar 15;119(2):795-800.
- PubMed ID
- 6424671 [ View in PubMed]
- Abstract
Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.