Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli.
Article Details
- CitationCopy to clipboard
Vasudevan SG, Tang P, Dixon NE, Poole RK
Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli.
FEMS Microbiol Lett. 1995 Jan 15;125(2-3):219-24.
- PubMed ID
- 7875569 [ View in PubMed]
- Abstract
The subcellular distribution of the soluble flavohaemoglobin (HMP) of Escherichia coli has been determined. Cells over-expressing HMP from the cloned hmp gene on a multicopy plasmid were fractionated by osmotic shock and lysozyme treatment. Spectral analysis of subcellular fractions showed the CO-binding haemoprotein to be cytoplasmic. However, Western blotting using antibody raised to purified HMP revealed approximately 30% of the protein to be periplasmic in the over-expressing strain. Western analysis also revealed substantial levels of periplasmic HMP in a strain expressing only chromosomally encoded protein but none in an hmp mutant. The results are discussed in relation to protein function and the similar distribution reported for Vitreoscilla globin.