Cloning and complete coding sequence of a novel human cathepsin expressed in giant cells of osteoclastomas.
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Li YP, Alexander M, Wucherpfennig AL, Yelick P, Chen W, Stashenko P
Cloning and complete coding sequence of a novel human cathepsin expressed in giant cells of osteoclastomas.
J Bone Miner Res. 1995 Aug;10(8):1197-202.
- PubMed ID
- 8585423 [ View in PubMed]
- Abstract
A gene encoding a possible novel human cathepsin, a cysteine proteinase that is distinct from previously characterized enzymes, has been identified by differential screening of a human osteoclastoma cDNA library. This molecule, termed cathepsin X, appears to represent the human homolog of the osteoclast-expressed rabbit cathepsin OC-2. Cathepsin X (GenBank accession number U20280) is 93.9% identical to OC-2 at the amino acid level, and is 92% identical at the nucleotide level within the coding region. Cathepsin X is 52.2 and 46.9% identical to cathepsins S and L, respectively, and is therefore clearly distinct from these enzymes. Cathepsin X mRNA was localized to multinucleated giant cells within the osteoclastoma tumor by in situ hybridization. These data strongly support the hypothesis that cathepsin X represents a novel cysteine proteinase which is expressed at high levels in osteoclasts.