Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans.
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Li Y, Ren S, Gong W
Cloning, high-level expression, purification and crystallization of peptide deformylase from Leptospira interrogans.
Acta Crystallogr D Biol Crystallogr. 2002 May;58(Pt 5):846-8. Epub 2002 Apr 26.
- PubMed ID
- 11976499 [ View in PubMed]
- Abstract
A new peptide deformylase (PDF; EC 3.5.1.27) gene from Leptospira interrogans was identified and cloned into expression plasmid pET22b(+) and was highly expressed in Escherichia coli BL21(DE3). With DEAE-Sepharose anion-exchange chromatography followed by Superdex G-75 size-exclusion chromatography, 60 mg of PDF from L. interrogans was purified from 1 l of cell culture. Crystallization screening of the purified enzyme resulted in two crystal forms, from one of which a 3 A resolution X-ray diffraction data set has been collected.