Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase.
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Sadlock JE, Lightowlers RN, Capaldi RA, Schon EA
Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase.
Biochim Biophys Acta. 1993 Feb 20;1172(1-2):223-5.
- PubMed ID
- 8382530 [ View in PubMed]
- Abstract
Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.