Amino Acid esters prevent thermal inactivation and aggregation of lysozyme.
Article Details
- CitationCopy to clipboard
Shiraki K, Kudou M, Sakamoto R, Yanagihara I, Takagi M
Amino Acid esters prevent thermal inactivation and aggregation of lysozyme.
Biotechnol Prog. 2005 Mar-Apr;21(2):640-3.
- PubMed ID
- 15801812 [ View in PubMed]
- Abstract
Small potent inhibitors of aggregation are eagerly demanded for preventing the inactivation of proteins. This paper shows that amino acid esters (AAEs) prevent heat-induced aggregation and inactivation of hen egg lysozyme. Lysozyme was completely inactivated (<1% original activity) during heat treatment at 98 degrees C for 30 min in a solution containing 0.2 mg/mL lysozyme in 50 mM Na-phosphate buffer (pH 6.5). The residual activities only slightly increased (<5%) in the presence of 100 mM commonly used additives such as arginine, guanidine, urea, and sugars. However, in the presence of 100 mM AAEs, the residual activities were >60% and no aggregates were observed during the heat treatment at 98 degrees C for 30 min. This fact provides new information on the scaffold for designing additives to prevent heat-induced aggregation.