Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase.
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Shanske S, Sakoda S, Hermodson MA, DiMauro S, Schon EA
Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase.
J Biol Chem. 1987 Oct 25;262(30):14612-7.
- PubMed ID
- 2822696 [ View in PubMed]
- Abstract
We have isolated a full-length cDNA specifying the muscle-specific subunit of human phosphoglycerate mutase (PGAM-M). The cDNA encodes a deduced protein 253 amino acids in length and contains an unusually short (37 nucleotides) 3'-untranslated region. The deduced human PGAM-M protein is clearly related to yeast PGAM and to human diphosphoglycerate mutase. Genomic Southern analysis using the PGAM-M cDNA as a probe implies the presence of a large PGAM gene family in the human genome, while Northern analysis demonstrates tissue-specific transcription of this isoenzyme gene.