Choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities.

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Hsieh BC, Matsumoto K, Cheng TJ, Yuu G, Chen RL

Choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities.

J Pharm Biomed Anal. 2007 Nov 30;45(4):673-8. Epub 2007 Jan 24.

PubMed ID
17329059 [ View in PubMed
]
Abstract

An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (<50.0 microm in thickness) was covalently immobilized with choline oxidase (EC 3.1.1.17 from Alcaligenes sp.) and then attached onto the platinum electrode of an amperometric flow cell. The flow cell served as the choline sensing device of the proposed FIA system. The sensor signal (peak height of the FIAgram) was linearly related to choline concentration (r=0.999 for choline up to 5.0mM) with low detection limit (S/N>3 for 10.0 microM choline) and high reproducibility (CV<3% for 1.0mM choline, n=7). The system was proved to be useful in measuring cholinesterase inhibitory activities of synthetic chemicals or natural products.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
CholineCholinesteraseProteinHumans
Unknown
Product of
Details
Choline salicylateCholinesteraseProteinHumans
Unknown
Product of
Details