A regulated RNA binding protein also possesses aconitase activity.
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- CitationCopy to clipboard
Kaptain S, Downey WE, Tang C, Philpott C, Haile D, Orloff DG, Harford JB, Rouault TA, Klausner RD
A regulated RNA binding protein also possesses aconitase activity.
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10109-13.
- PubMed ID
- 1946430 [ View in PubMed]
- Abstract
A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.