Cell adhesion receptor GPR133 couples to Gs protein.
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Bohnekamp J, Schoneberg T
Cell adhesion receptor GPR133 couples to Gs protein.
J Biol Chem. 2011 Dec 9;286(49):41912-6. doi: 10.1074/jbc.C111.265934. Epub 2011 Oct 24.
- PubMed ID
- 22025619 [ View in PubMed]
- Abstract
Adhesion G protein-coupled receptors (GPCR), with their very large and complex N termini, are thought to participate in cell-cell and cell-matrix interactions and appear to be highly relevant in several developmental processes. Their intracellular signaling is still poorly understood. Here we demonstrate that GPR133, a member of the adhesion GPCR subfamily, activates the G(s) protein/adenylyl cyclase pathway. The presence of the N terminus and the cleavage at the GPCR proteolysis site are not required for G protein signaling. G(s) protein coupling was verified by Galpha(s) knockdown with siRNA, overexpression of Galpha(s), co-expression of the chimeric Gq(s4) protein that routes GPR133 activity to the phospholipase C/inositol phosphate pathway, and missense mutation within the transmembrane domain that abolished receptor activity without changing cell surface expression. It is likely that not only GPR133 but also other adhesion GPCR signal via classical receptor/G protein-interaction.