Integrin-dependent tyrosine phoshorylation and cytoskeletal translocation of Tec in thrombin-activated platelets.
Article Details
- CitationCopy to clipboard
Laffargue M, Monnereau L, Tuech J, Ragab A, Ragab-Thomas J, Payrastre B, Raynal P, Chap H
Integrin-dependent tyrosine phoshorylation and cytoskeletal translocation of Tec in thrombin-activated platelets.
Biochem Biophys Res Commun. 1997 Sep 8;238(1):247-51.
- PubMed ID
- 9299487 [ View in PubMed]
- Abstract
Using a specific polyclonal anti-Tec antibody, we have shown that Tec is expressed in human platelets. In addition, Tec was found to undergo tyrosine phosphorylation during platelet activation. The phosphorylation increased after 1 min and remained stable after 3 min of thrombin treatment. The tetrapeptide RGDS inhibited more than 90% of thrombin-induced tyrosine phosphorylation of Tec and blocked its translocation to the cytoskeleton. These results suggest that Tec participates in platelet signaling downstream of integrin activation.