Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins.
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Miyamoto K, Sakurai H, Sugiura T
Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins.
Proteomics. 2008 Jul;8(14):2907-10. doi: 10.1002/pmic.200800083.
- PubMed ID
- 18655028 [ View in PubMed]
- Abstract
RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events.