Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver.
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Ritonja A, Machleidt W, Barrett AJ
Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver.
Biochem Biophys Res Commun. 1985 Sep 30;131(3):1187-92.
- PubMed ID
- 3902020 [ View in PubMed]
- Abstract
The complete amino acid sequence of the cysteine proteinase inhibitor cystatin B (formerly named CPI-B) from human liver was determined. The 98-residue sequence (Mr = 11,175) was obtained by automated solid-phase Edman degradation of a large cyanogen bromide fragment and peptides generated by enzymatic cleavage. The protein starts with a blocked Met-Met sequence which is presumably N-acetylated. The sequence shows that human cystatin B is a member of the family of intracellular cystatins; it is 79% identical with cystatin beta from rat liver, but contains only a single cysteine. Human cystatin B is able to form a dimer stabilized by noncovalent forces.