A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator.
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Nelbock P, Dillon PJ, Perkins A, Rosen CA
A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator.
Science. 1990 Jun 29;248(4963):1650-3.
- PubMed ID
- 2194290 [ View in PubMed]
- Abstract
The human immunodeficiency virus (HIV) tat protein (Tat) is a positive regulator of virus gene expression and replication. Biotinylated Tat was used as a probe to screen a lambda gt11 fusion protein library, and a complementary DNA encoding a protein that interacts with Tat was cloned. Expression of this protein, designated TBP-1 (for Tat binding protein-1), was observed in a variety of cell lines, with expression being highest in human cells. TBP-1 was localized predominantly in the nucleus, which is consistent with the nuclear localization of Tat. In cotransfection experiments, expression of TBP-1 was able to specifically suppress Tat-mediated transactivation. The strategy described may be useful for direct identification and cloning of genes encoding proteins that associate with other proteins to modulate their activity in a positive or negative fashion.