Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication.
Article Details
- CitationCopy to clipboard
Boel E, Vuust J, Norris F, Norris K, Wind A, Rehfeld JF, Marcker KA
Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication.
Proc Natl Acad Sci U S A. 1983 May;80(10):2866-9.
- PubMed ID
- 6574456 [ View in PubMed]
- Abstract
An oligo(dT)-primed cDNA copy of the mRNA coding for the human gastrin precursor was constructed from poly(A)-containing RNA from a human pancreatic, gastrin-producing tumor (a gastrinoma). The cDNA was inserted into the Pst I endonuclease site of plasmid pBR322 by the use of the poly(dC) and poly(dG) tailing procedure. Clones containing gastrin sequences were selected by hybridization to a purified single-stranded 32P-labeled gastrin cDNA probe. This probe was constructed with gastrinoma mRNA as template. As primer for the cDNA synthesis, we used a synthetic oligonucleotide mixture, d(AG-A-A-AG-T-C-C-A-T-C-C-A), corresponding to the gastrin-specific amino acid sequence Trp-Met-Asp-Phe. In this way we determined the nucleotide sequence of the entire coding region (303 nucleotides), the entire 3' untranslated region (102 nucleotides), and 8 nucleotides of the 5' untranslated region. A striking homology between parts of the coding region suggests that evolution of the gastrin gene has involved a gene duplication.