Structural and functional analysis of the 5'-flanking region of the human insulin-like growth factor binding protein (IGFBP)-4 gene.
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Qin X, Morales S, Lee KW, Boonyaratanakornkit V, Baylink DJ, Mohan S, Strong DD
Structural and functional analysis of the 5'-flanking region of the human insulin-like growth factor binding protein (IGFBP)-4 gene.
Biochim Biophys Acta. 1997 Feb 7;1350(2):136-40.
- PubMed ID
- 9048882 [ View in PubMed]
- Abstract
More than 3 kb of the human (h)IGFBP-4 gene 5'-flanking region was sequenced and assessed for promoter activity. The hIGFBP-4 promoter resides within a CpG island and demonstrates strong basal activity in human osteoblast-like osteosarcoma and COS-7 monkey kidney cells. Transient transfection of cells with hIGFBP-4 promoter-linked deletion constructs demonstrated that multiple cooperating cis-acting elements within 836 bp of the 5'-flanking region contributed to overall promoter strength.