The N-terminus domain of the a2 isoform of vacuolar ATPase can regulate interleukin-1beta production from mononuclear cells in co-culture with JEG-3 choriocarcinoma cells.
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Ntrivalas E, Gilman-Sachs A, Kwak-Kim J, Beaman K
The N-terminus domain of the a2 isoform of vacuolar ATPase can regulate interleukin-1beta production from mononuclear cells in co-culture with JEG-3 choriocarcinoma cells.
Am J Reprod Immunol. 2007 Mar;57(3):201-9. doi: 10.1111/j.1600-0897.2006.00463.x.
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- 17295899 [ View in PubMed]
- Abstract
PROBLEM: a2V-ATPase is the a2 isoform of vacuolar ATPase and is expressed in human trophoblast cells. a2V-ATPase resides as a 70-kDa molecule in intracellular vesicles. Upon cell stimulation, it migrates to the surface as a 50-kDa molecule, after a 20-kDa portion [N-terminus domain of the a2V-ATPase (a2NTD)] is cleaved and secreted to the extracellular environment. Previous studies showed that a2NTD-regulated cytokine production from stimulated T cells. The aim of this study was to determine if a2NTD can regulate cytokine production from immune cells that were in contact with JEG-3 cells. METHOD OF STUDY: Peripheral blood mononuclear cells (PBMC) from females were co-cultured with JEG-3 cells in the presence or absence of a2NTD, and supernatants were analyzed by enzyme-linked immunosorbent assay for interleukin (IL)-1beta. Additionally, PBMC cultured with JEG-3 cells, in the presence or absence of a2NTD, were analyzed for cytokine gene expression by gene arrays. RESULTS: There was an increased secretion of IL-1beta and a decrease in type I and II IL-1 receptors (IL1RA and IL-1R2) gene expression in PBMC that were co-cultured with JEG-3 cells in the presence of a2NTD. CONCLUSION: These data suggest a role for a2NTD in the regulation of IL-1beta pro-inflammatory cytokine production at the fetal-maternal interface.