Ligand-directed tosyl chemistry for protein labeling in vivo.
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Tsukiji S, Miyagawa M, Takaoka Y, Tamura T, Hamachi I
Ligand-directed tosyl chemistry for protein labeling in vivo.
Nat Chem Biol. 2009 May;5(5):341-3. doi: 10.1038/nchembio.157. Epub 2009 Mar 29.
- PubMed ID
- 19330012 [ View in PubMed]
- Abstract
Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
DrugBank Data that Cites this Article
- Binding Properties
Drug Target Property Measurement pH Temperature (°C) Ethoxzolamide Carbonic anhydrase 1 Ki (nM) 25 N/A N/A Details