Development and characterization of new inhibitors of the human and mouse hematopoietic prostaglandin D(2) synthases.

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Christ AN, Labzin L, Bourne GT, Fukunishi H, Weber JE, Sweet MJ, Smythe ML, Flanagan JU

Development and characterization of new inhibitors of the human and mouse hematopoietic prostaglandin D(2) synthases.

J Med Chem. 2010 Aug 12;53(15):5536-48. doi: 10.1021/jm100194a.

PubMed ID

20684598 [ View in PubMed

]

Abstract

The hematopoietic prostaglandin D(2) synthase has a proinflammatory effect in a range of diseases, including allergic asthma, where its product prostaglandin D(2) (PGD(2)) has a role in regulating many of the hallmark disease characteristics. Here we describe the development and characterization of a novel series of hematopoietic prostaglandin D(2) synthase inhibitors with potency similar to that of known inhibitors. Compounds N-benzhydryl-5-(3-hydroxyphenyl)thiophene-2-carboxamide (compound 8) and N-(1-amino-1-oxo-3-phenylpropan-2-yl)-6-(thiophen-2-yl)nicotinamide (compound 34) demonstrated low micromolar potency in the inhibition of the purified enzyme, while only 34 reduced Toll-like receptor (TLR) inducible PGD(2) production in both mouse primary bone marrow-derived macrophages and the human megakaryocytic cell line MEG-01S. Importantly, 34 demonstrated a greater selectivity for inhibition of PGD(2) synthesis versus other eicosanoids that lie downstream of PGH(2) (PGE(2) and markers of prostacyclin (6-keto PGF(1alpha)) and thromboxane (TXB(2))) when compared to the known inhibitors HQL-79 (compound 1) and 2-phenyl-5-(1H-pyrazol-3-yl)thiazole (compound 2). Compound 34 therefore represents a selective hematopoietic prostaglandin D(2) synthase inhibitor.

DrugBank Data that Cites this Article

Binding Properties

Drug	Target	Property	Measurement	pH	Temperature (°C)
4-{[4-(4-fluoro-3-methylphenyl)-1,3-thiazol-2-yl]amino}-2-hydroxybenzoic acid	Hematopoietic prostaglandin D synthase	IC 50 (nM)	1400	N/A	N/A	Details
4-{[4-(4-fluoro-3-methylphenyl)-1,3-thiazol-2-yl]amino}-2-hydroxybenzoic acid	Hematopoietic prostaglandin D synthase	IC 50 (nM)	94600	N/A	N/A	Details
PHENYL-5-(1H-PYRAZOL-3-YL)-1,3-THIAZOLE	Hematopoietic prostaglandin D synthase	IC 50 (nM)	700	N/A	N/A	Details
PHENYL-5-(1H-PYRAZOL-3-YL)-1,3-THIAZOLE	Hematopoietic prostaglandin D synthase	IC 50 (nM)	97700	N/A	N/A	Details