Isolation of recombinant BMP receptor IA ectodomain and its 2:1 complex with BMP-2.

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Kirsch T, Nickel J, Sebald W

Isolation of recombinant BMP receptor IA ectodomain and its 2:1 complex with BMP-2.

FEBS Lett. 2000 Feb 25;468(2-3):215-9.

PubMed ID

10692589 [ View in PubMed

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Abstract

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta superfamily which induces bone formation and regeneration, and important steps during early embryonic development. BMP-2 signals via oligomerization of type I and type II serine/threonine kinase receptors. We report here expression of the extracellular domain of the human type IA receptor for BMP-2 (BMPR-IA) in Escherichia coli. This soluble form of BMPR-IA (sBMPR-IA) was purified employing a BMP-2 affinity column. Gel filtration experiments and analysis of gel filtration fractions by polyacrylamide electrophoresis and densitometry reveal that BMP-2 forms a defined 1:2 complex with sBMPR-IA that can be purified and hopefully used for crystallization studies.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Dibotermin alfa	Bone morphogenetic protein receptor type-1A	Protein	Humans	Yes	Ligand	Details