Isolation and characterization of a human thiamine pyrophosphokinase cDNA.
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Nosaka K, Onozuka M, Kakazu N, Hibi S, Nishimura H, Nishino H, Abe T
Isolation and characterization of a human thiamine pyrophosphokinase cDNA.
Biochim Biophys Acta. 2001 Jan 26;1517(2):293-7.
- PubMed ID
- 11342111 [ View in PubMed]
- Abstract
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.